Periodic binding of troponin C•I and troponin I to tropomyosin-actin filaments
Periodic binding of troponin C•I and troponin I to tropomyosin-actin filaments
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DOI：
10.1093/oxfordjournals.jbchem.a003159
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发表时间：
2002-05-01
影响因子：
2.7
通讯作者：
Shiraishi, F
中科院分区：
文献类型：
--
作者：
Ohtsuki, I;Shiraishi, F
我们用免疫电镜观察了肌钙蛋白C.I和肌钙蛋白I沿着原肌球蛋白-肌动蛋白纤维的分布，发现抗肌钙蛋白I抗体沿肌钙蛋白C. I-原肌球蛋白-肌动蛋白和肌钙蛋白I-原肌球蛋白-肌动蛋白纤维束形成间隔为沿着38 nm的横纹。由于38 nm的长度对应于丝状原肌球蛋白沿着肌动蛋白双链的重复周期，本研究表明，肌钙蛋白I位于每个原肌球蛋白的特定区域，这表明肌钙蛋白I和原肌球蛋白之间的特定相互作用参与决定肌钙蛋白I沿着原肌球蛋白-肌动蛋白丝的周期性分布。
We investigated the distribution of troponin C.I and troponin I along tropomyosin-actin filaments by immunoelectron microscopy and found that anti-troponin I antibody formed transverse striations at 38 nm intervals along the bundle of filaments of both troponin C.I-tropomyosin-actin and troponin I-tropomyosin-actin. Since the length of 38 nm corresponds to the repeating period of filamentous tropomyosin along actin double strands, the present study indicates that troponin I is located at a specific region of each tropomyosin, suggesting that a specific interaction between troponin I and tropomyosin is involved in determining the periodic distribution of troponin I along tropomyosin-actin filaments.