microRNA-192,-194 and-215 are frequently downregulated in colorectal cancer
microRNA-192,-194 and-215 are frequently downregulated in colorectal cancer
复制标题
DOI：
10.3892/etm.2011.436
复制
发表时间：
2012-03-01
影响因子：
2.7
通讯作者：
Xu, Huimian
中科院分区：
文献类型：
--
作者：
Chiang, Yeunpo;Song, Yongxi;Xu, Huimian
MicroRNAs(MiRNAs)是一种小的、内源性的非编码RNA。越来越多的研究表明，它们在许多肿瘤类型中都有异常表达。MIR-192、-194和-215尚未利用大量结直肠癌(CRC)病例进行全面调查。我们从107例结直肠癌组织和3个结直肠癌细胞系中提取总RNA。用实时定量聚合酶链式反应(Real-time PCR)方法检测miR-192、-194和-215的表达水平，以评价miR-192、-194和-215的表达水平与临床病理特征的关系。最后，通过四甲基偶氮唑盐比色法研究miR-194对HCT-116细胞增殖的影响。MIR-192、-194和-215在结直肠癌组织中表达显著下调(P
microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p