Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis
Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis
复制标题
DOI：
10.1104/pp.102.016444
复制
发表时间：
2003-04-01
期刊：
影响因子：
7.4
通讯作者：
Hahn, MG
中科院分区：
文献类型：
--
作者：
Freshour, G;Bonin, CP;Hahn, MG
单克隆抗体 CCRC-M1 可识别主要存在于细胞壁多糖木葡聚糖中的含岩藻糖 (Fuc) 的表位，用于确定该表位在拟南芥 mur1 突变体中的分布。整个幼苗的免疫荧光标记显示，mur1 根毛被 CCRC-M1 严重染色，而根体保持未染色或仅轻微染色。免疫金标记显示，mur1 根内的 CCRC-M1 标记对特定细胞壁和细胞类型具有特异性。 CCRC-M1 标记 2 d 及以上初生根顶端和成熟侧根顶端的所有细胞壁，但不与侧根首字母的细胞壁结合。用 CCRC-M1 标记会减少正在经历快速伸长生长的 mur1 根细胞，使得在初生根和侧根的成熟部分中，仅标记中柱鞘细胞壁和表皮细胞外壁。突变体在含有 Fuc 的培养基上生长会恢复野生型标记，其中所有细胞壁都被 CCRC-M1 抗体标记。 Mur1 下胚轴、芽或叶中未观察到标记；托叶被标记。 CCRC-M1 确实标记花药和花粉管壁内的花粉粒。这些结果表明，用于掺入木葡聚糖的 Fuc 是使用 GDP-D-甘露糖 4,6-脱水酶的一种或另一种或两种亚型合成的，具体取决于细胞类型和/或细胞的发育状态。
The monoclonal antibody, CCRC-M1, which recognizes a fucose (Fuc)-containing epitope found principally in the cell wall polysaccharide xyloglucan, was used to determine the distribution of this epitope throughout the mur1 mutant of Arabidopsis. Immunofluorescent labeling of whole seedlings revealed that mur1 root hairs are stained heavily by CCRC-M1, whereas the body of the root remains unstained or only lightly stained., Immunogold labeling showed that CCRC-M1 labeling within the mur1 root is specific to particular cell walls and cell types. CCRC-M1 labels all cell walls at the apex of primary roots 2 d and older and the apices of mature lateral roots, but does not bind to cell walls in lateral root initials. Labeling with CCRC-M1 decreases in mur1 root cells that are undergoing rapid elongation growth such that, in the mature portions of primary and lateral roots, only the walls of pericycle cells and the outer walls of epidermal cells are labeled. Growth of the mutant on Fuc-containing media restores wild-type labeling, where all cell walls are labeled by the CCRC-M1 antibody. No labeling was observed in mur1 hypocotyls, shoots, or leaves; stipules are labeled. CCRC-M1 does label pollen grains within anthers and pollen tube walls. These results suggest the Fuc destined for incorporation into xyloglucan is synthesized using one or the other or both isoforms of GDP-D-mannose 4,6-dehydratase, depending on the cell type and/or developmental state of the cell.