Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue.
Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue.
复制标题
从正常人乳腺组织中纯化不同的上皮细胞亚群。
DOI：
10.1007/978-1-4939-6475-8_13
复制
发表时间：
2017
影响因子：
--
通讯作者：
J. Stingl
中科院分区：
文献类型：
--
作者：
Mona Shehata;J. Stingl
乳腺上皮由多种特殊类型的细胞组成，这些细胞协调运作，在多个妊娠周期中产生和排出乳汁。识别和纯化这些细胞亚群的能力，以询问它们的生长和分化能力，以及表征调节它们行为的分子机制，对于识别与乳腺癌发生和发展相关的过程是至关重要的。本章概述了将人类乳房缩小标本分离到活细胞的单细胞悬浮液的分步方法。此外，还描述了通过使用荧光激活细胞分选来纯化四种不同的上皮细胞亚群的策略，以及在贴壁培养中以克隆密度询问这些纯化细胞的生长和分化特性的方案。
The mammary epithelium is composed of a variety of specialized cell types that function in a coordinated fashion to produce and eject milk through multiple cycles of pregnancy. The ability to identify and purify these subsets of cells in order to interrogate their growth and differentiation capacities, as well as to characterize the molecular mechanisms that regulate their behavior, is essential in identifying the processes associated with breast cancer initiation and progression. This methods chapter outlines the step-by-step methods for dissociating human breast reduction specimens to a single cell suspension of viable cells. As well, strategies for purifying four distinct subsets of epithelial cells by using fluorescence-activated cell sorting and protocols for interrogating the growth and differentiation properties of these purified cells at clonal densities in adherent culture are also described.
影响因子：
23.9
作者：
Ginestier, Christophe;Hur, Min Hee;Dontu, Gabriela
通讯作者：
Dontu, Gabriela