Eukaryotic Community Shift in Response to Organic Loading Rate of an Aerobic Trickling Filter (Down-Flow Hanging Sponge Reactor) Treating Domestic Sewage
Eukaryotic Community Shift in Response to Organic Loading Rate of an Aerobic Trickling Filter (Down-Flow Hanging Sponge Reactor) Treating Domestic Sewage
复制标题
处理生活污水的好氧滴滤池（下流式悬挂海绵反应器）的有机负荷率引起的真核群落变化
DOI：
10.1007/s00248-016-0871-0
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发表时间：
2017
影响因子：
3.6
通讯作者：
and Syutsubo K
中科院分区：
文献类型：
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作者：
Miyaoka Y;Hatamoto M;Yamaguchi T;and Syutsubo K
研究了在不同有机负荷率（OLRs）下，好氧滴滤塔（降流式悬挂海绵，DHS）处理生活污水过程中真核生物群落结构和水质的变化。OLR明显影响海绵孔隙水质量和相对鞭毛虫和纤毛虫（自由游泳，食肉，爬行，跟踪原生动物）丰度保留污泥。在OLR从1.05增加到1.97 kg化学需氧量（COD）m-3day-1后，COD和NH 4 +-N的处理效率都下降，并且在DHS反应器的中底部，鞭毛虫和纤毛虫的相对丰度从2- 8%增加到51- 65%。在稳定OLR（2.01 kg COD m−3day−1）的连续运行中，出水水质得到改善，DHS反应器中底部的相对鞭毛虫和纤毛虫丰度降至总细胞的15- 46%。这一结果可能表明鞭毛虫和纤毛虫优先捕食分散的细菌，从而稳定出水水质。此外，为了研究真核生物群落结构，构建了基于保留污泥的18 S核糖体核糖核酸（rRNA）基因的克隆文库。占优势的组是核霉菌属（Nucletmycea）的三型，占总克隆的约29- 56%。此外，大部分克隆在NCBI数据库中具有<97%的序列同一性。该结果表明，DHS反应器中存在遗传学未知的真核生物。这些结果为DHS反应器处理生活污水中真核生物群落的转变提供了依据。
In this study, changes in eukaryotic community structure and water quality were investigated in an aerobic trickling filter (down-flow hanging sponge, DHS) treating domestic sewage under different organic loading rates (OLRs). The OLR clearly influenced both sponge pore water quality and relative flagellates and ciliates (free-swimming, carnivorous, crawling, and stalked protozoa) abundances in the retained sludge. Immediately after the OLR was increased from 1.05 to 1.97 kg chemical oxygen demand (COD) m−3day−1, COD and NH4+-N treatment efficiencies both deteriorated, and relative flagellates and ciliates abundances then increased from 2–8 % to 51–65 % total cells in the middle-bottom part of the DHS reactor. In a continuous operation at a stable OLR (2.01 kg COD m−3day−1), effluent water quality improved, and relative flagellates and ciliates abundances decreased to 15–46 % total cells in the middle-bottom part of the DHS reactor. This result may indicate that flagellates and ciliates preferentially graze on dispersed bacteria, thus, stabilizing effluent water quality. Additionally, to investigate eukaryotic community structure, clone libraries based on the 18S ribosomal ribonucleic acid (rRNA) gene of the retained sludge were constructed. The predominant group was Nucletmycea phylotypes, representing approximately 29–56 % total clones. Furthermore, a large proportion of the clones had <97 % sequence identity in the NCBI database. This result indicates that phylogenetically unknown eukaryotes were present in the DHS reactor. These results provide insights into eukaryotic community shift in the DHS reactor treating domestic sewage.