Myosin-V Opposes Microtubule-Based Cargo Transport and Drives Directional Motility on Cortical Actin
Myosin-V Opposes Microtubule-Based Cargo Transport and Drives Directional Motility on Cortical Actin
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DOI：
10.1016/j.cub.2013.03.068
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发表时间：
2013-05-06
期刊：
影响因子：
9.2
通讯作者：
Hoogenraad, Casper C.
中科院分区：
文献类型：
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作者：
Kapitein, Lukas C.;van Bergeijk, Petra;Hoogenraad, Casper C.
细胞内运输由马达蛋白驱动，马达蛋白使用微管或肌动蛋白丝作为其轨道[1]，但这些运输途径之间的相互作用知之甚少[2-4]。尽管已知许多基于微管的马达驱动长距离运输，但已经提出了几种基于肌动蛋白的马达主要用于货物系留[4-6]。这些相反的活动是如何在包含两种类型的发动机的货物上集成的尚不清楚。在这里，我们使用诱导的细胞内转运试验表明，急性招募肌球蛋白-V驱动蛋白推进货物降低其运动性附近的细胞周边，并提高其本地化的肌动蛋白丰富的细胞皮质。肌球蛋白-V阻止基于微管的快速运输，而不需要调节驱动蛋白马达的自动或其他抑制。此外，肌球蛋白-V，尽管是一个无效的远程转运，可以驱动缓慢，中程（1-5 μ m），点对点运输皮质细胞区域。总之，这些数据支持一个模型，其中肌球蛋白-V建立局部皮质传递的驱动蛋白结合货物通过结合拴系和主动运输。
Intracellular transport is driven by motor proteins that either use microtubules or actin filaments as their tracks [1], but the interplay between these transport pathways is poorly understood [2-4]. Whereas many microtubule-based motors are known to drive long-range transport, several actin-based motors have been proposed to function predominantly in cargo tethering [4-6]. How these opposing activities are integrated on cargoes that contain both types of motors is unknown. Here we use inducible intracellular transport assays to show that acute recruitment of myosin-V to kinesin-propelled cargo reduces their motility near the cell periphery and enhances their localization at the actin-rich cell cortex. Myosin-V arrests rapid microtubule-based transport without the need for regulated auto- or other inhibition of kinesin motors. In addition, myosin-V, despite being an ineffective long-range transporter, can drive slow, medium-range (1-5 mu m), point-to-point transport in cortical cell regions. Altogether, these data support a model in which myosin-V establishes local cortical delivery of kinesin-bound cargos through a combination of tethering and active transport.