Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument
Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument
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DOI：
10.1016/s1044-0305(98)00126-3
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发表时间：
1999-01-01
影响因子：
3.2
通讯作者：
Caprioli, RM
中科院分区：
文献类型：
--
作者：
Stoeckli, M;Farmer, TB;Caprioli, RM
自动使用的基质辅助激光解吸电离（MALDI）质谱仪（MS）的图像分析的样品，通过实施新的软件进行仪器控制，数据采集和数据分析。该软件允许自动采集MS MALDI谱以形成有序数据阵列，并包含显示功能以提供一个或多个质荷比值的图像。该技术可用于扫描组织样本、印迹样本、凝胶或其他需要对样本进行图像分析的样本表面。该程序实现了每个图像点通常1秒的时间，允许分析由大量的点组成，具有高达850 dpi的高空间分辨率。该软件的功能在本文中展示了打印图像的样本，其中可见光图像可以与质谱法获得的图像进行比较。通过分析一系列含有不同量的几种蛋白质的样品点来引入定量方面。（J Am. Soc Mass Spectrom 1999，10，67-71）（C）1999美国质谱学会。
The automated use of a matrix-assisted laser desorption ionization (MALDI) mass spectrometer (MS) is described for image analysis of samples through implementation of new software for instrument control, data acquisition, and data analysis. The software permits automated acquisition of MS MALDI spectra to form an ordered data array and contains display features to provide images at one or more mass-to-charge ratio values. The technique can be used to scan tissue samples, blotted samples, gels, or other sample surfaces where the image analysis of that sample is required. The program achieves a time of typically 1 s per image point, permitting an analysis made up of large numbers of points with high spatial resolution up to 850 dpi. The features of the software are demonstrated in this paper with samples of printed images, where visible images can be compared to those obtained by mass spectrometry. Quantitative aspects are introduced by analyzing a series of sample spots containing different amounts of several proteins. (J Am. Soc Mass Spectrom 1999, 10, 67-71) (C) 1999 American Society for Mass Spectrometry.