Oscillations of the transepithelial potential of moth olfactory sensilla are influenced by octopamine and serotonin.
Oscillations of the transepithelial potential of moth olfactory sensilla are influenced by octopamine and serotonin.
复制标题
飞蛾嗅觉感受器跨上皮电位的振荡受章鱼胺和血清素的影响。
DOI：
--
复制
发表时间：
2001
影响因子：
2.8
通讯作者：
M. Stengl
中科院分区：
文献类型：
--
作者：
J. Dolzer;S. Krannich;K. Fischer;M. Stengl
生物胺章鱼胺是已知的，以提高敏感性的雄蛾，他们的物种特异性信息素在飞行隧道实验。这种信息素引导的逆风飞行的敏化至少部分是由于嗅觉受体神经元的信息素响应的峰值神经脉冲频率的章鱼胺依赖性增加。然而，目前尚不清楚章鱼胺是否直接对嗅觉受体神经元的电特性产生影响，或者通过调节感觉器的辅助细胞间接地产生影响。在雄天蛾触角上的信息素依赖性的触角感觉器的细胞外尖端记录中，我们研究了章鱼胺和5-羟色胺对感觉器辅助细胞中V-ATP酶活性产生的跨上皮电位的影响。此外，未受刺激的嗅觉受体神经元的动作电位活动进行了检查，在生物胺的存在下。在恒定的环境条件下，跨上皮电位有规律地振荡，周期为2-8分钟，峰-峰幅度为1- 25 mV，持续数小时。这些振荡的间隔被中断期间相对稳定的跨上皮电位，与飞行活动的蛾。章鱼胺以剂量依赖性方式降低跨上皮电位振荡的幅度，并降低感器制剂的电阻。5-羟色胺改变了跨上皮电位的波形，但没有改变制剂的电阻。因此，这两种胺影响辅助细胞，但在跨上皮电位的调节中具有不同的靶点。这两种胺都不显着影响嗅觉受体神经元的自发动作电位活动。
The biogenic amine octopamine is known to enhance the sensitivity of male moths to their species-specific pheromones in flight-tunnel experiments. This sensitization of pheromone-guided upwind flight is at least partly due to octopamine-dependent increases in the peak nerve impulse frequency of the pheromone response of olfactory receptor neurons. It is not known, however, whether octopamine exerts its effects directly on the electrical properties of the olfactory receptor neurons or indirectly, via modulation of the accessory cells of the sensillum. In extracellular tip recordings of pheromone-dependent trichoid sensilla on the antennae of male Manduca sexta moths, we investigated the effects of octopamine and serotonin on the transepithelial potential, which is generated by the activity of V-ATPases in sensillar accessory cells. In addition, the action potential activity of unstimulated olfactory receptor neurons was examined in the presence of biogenic amines. Under constant environmental conditions, the transepithelial potential oscillated regularly with periods of 2-8 min and with a 1-25mV peak-to-peak amplitude over periods of several hours. These oscillatory intervals were interrupted by periods of relatively stable transepithelial potential, correlated with flight activity by the moth. Octopamine reduced the amplitude of the transepithelial potential oscillation and decreased the resistance of the sensillum preparation in a dose-dependent manner. Serotonin altered the waveform of the transepithelial potential, but did not change the resistance of the preparation. Thus, both amines affect the accessory cells, but have different targets in the regulation of the transepithelial potential. Neither amine significantly influenced the spontaneous action potential activity of the olfactory receptor neurons.
昆虫 V-ATP 酶，一种质膜质子泵，为次级主动运输提供能量：烟草天蛾中肠中电钾运输的分子分析。
DOI：
10.1242/jeb.172.1.335
发表时间：
1992
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Wieczorek,H
通讯作者：
Wieczorek,H
昆虫质膜H()V-ATP酶的结构和调控。
DOI：
10.1242/jeb.203.1.127
发表时间：
2000
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Wieczorek,H;Grber,G;Harvey,WR;Huss,M;Merzendorfer,H;Zeiske,W
通讯作者：
Zeiske,W
通过化学渗透 H V-ATP 酶为动物质膜供能。
DOI：
10.1242/jeb.200.2.203
发表时间：
1997
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Harvey,WR;Wieczorek,H
通讯作者：
Wieczorek,H