Fluorescent imaging of citrate and other intermediates in the citric acid cycle

Fluorescent imaging of citrate and other intermediates in the citric acid cycle
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DOI:
10.1002/anie.200353169
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发表时间:
2004-03-19
影响因子:
16.6
通讯作者:
Wolfbeis, OS
Wolfbeis, OS
中科院分区:
化学1区
文献类型:
--
作者:
Lin, ZH;Wu, M;Wolfbeis, OS

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了解情况:柠檬酸盐和其他柠檬酸循环的中间体可以通过Eu3+四环素络合物作为荧光探针的时间分辨荧光进行成像(见图)。时间分辨率使中间体之间能够区分,不需要酶转化。
Getting the picture: Citrate and other intermediates of the citric acid cycle can be imaged by time-resolved fluorescence with a Eu 3+ tetracycline complex as a fluorescent probe (see picture). The time resolution enables discrimination between intermediates, and enzymatic conversions are not needed.