Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity
Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity
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DOI：
10.1016/j.meegid.2011.01.004
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发表时间：
2013-04-01
影响因子：
3.2
通讯作者：
Buchy, Philippe
中科院分区：
文献类型：
--
作者：
Duong, Veasna;Trinh Thi Xuan Mai;Buchy, Philippe
森林斑疹伤寒是一种急性传染病，由一种独有的细胞内东方菌引起，该细菌是由细毛虫属受感染的恙螨叮咬后引起的。这种人畜共患疾病是亚太地区发热性疾病的主要原因，临床表现多种多样，从不明显的或轻微的疾病到致命的疾病。恙虫病原虫具有很高的基因组可塑性和菌株间大量的抗原性变异。以编码主要抗原蛋白的56 kDa类型特异性抗原(TSA)基因为参照，研究了流行株与流行株间的亲缘关系。对来自患者样本的41个序列(28个柬埔寨株和13个越南株)的56 kDa TSA基因的开放阅读框进行了测序，并用于基因分型。28株柬埔寨分离株聚为5大类群，分别为Karp(43.5%)、JG-v(25%)、Kato/TA716(21.5%)、TA763(3.5%)和Gilliam(3.5%)。在13株越南分离株中检出Karp(77%)、TA763(15.5%)和JG-v(7.5%)菌株。这是柬埔寨和越南中部首次在全国范围内进行基因分型描述。这些结果表明，在两国的共同循环中，基因类型存在相当大的差异。基因分型结果可能会提高柬埔寨和越南临床医生对流行株的高度遗传多样性的认识，为斑疹伤寒感染的血清学和分子诊断提供独特和有益的数据，并为未来的研究和疫苗开发提供原料。(C)2011爱思唯尔B.V.保留所有权利。
Scrub typhus is an acute infectious disease caused by an obligate intracellular bacterium Orientia tsutsugamushi following the bite of infected trombiculid mites of the genus Leptotrombidium. This zoonotic disease is a major cause of febrile illness in the Asia-Pacific region, with a large spectrum of clinical manifestations from unapparent or mild disease to fatal disease. O. tsutsugamushi is characterized by a very high genomic plasticity and a large number of antigenic variants amongst strains. The 56-kDa type specific antigen (TSA) gene, encoding the major antigenic protein, was used as reference to investigate the genetic relationships between the strains and to genotype O. tsutsugamushi isolates. The open reading frame of the 56-kDa TSA gene of 41 sequences (28 Cambodian and 13 Vietnamese strains) from patient samples were sequenced and used for genotyping. The 28 Cambodian isolates clustered into 5 major groups, including Karp (43.5%), JG-v (25%), Kato/TA716 (21.5%), TA763 (3.5%) and Gilliam (3.5%). Karp (77%), TA763 (15.5%) and JG-v (7.5%) strains were identified amongst the 13 Vietnamese isolates. This is the first countrywide genotyping description in Cambodia and in Central Vietnam. These results demonstrate the considerable diversity of genotypes in co-circulation in both countries. The genotyping result might raise awareness amongst Cambodian and Vietnamese clinicians of the high genetic diversity of circulating O. tsutsugamushi strains and provides unique and beneficial data for serological and molecular diagnosis of scrub typhus infections as well as raw materials for future studies and vaccine development. (C) 2011 Elsevier B.V. All rights reserved.