Cytokine expression patterns distinguish HIV associated skin diseases.
Cytokine expression patterns distinguish HIV associated skin diseases.
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细胞因子表达模式可区分 HIV 相关皮肤病。
DOI：
10.1034/j.1600-0625.2000.009005341.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Duvic,M
中科院分区：
文献类型：
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作者：
Breuer-McHam,JN;Ledbetter,LS;Sarris,AH;Duvic,M
众所周知，艾滋病会引起外周细胞因子的变化。然而，HIV相关皮肤病患者皮肤中的主要细胞因子尚未明确定义。我们假设存在不同的细胞因子谱来区分艾滋病相关皮肤病的不同临床表现。为了验证这一假设，我们对53例卡波西氏肉瘤（KS）、银屑病和嗜酸性毛囊炎引起的瘙痒的HIV阳性患者以及治疗前银屑病或KS的HIV阴性对照进行了病变和非病变皮肤活检。用肿瘤坏死因子（TNF）‐α、白细胞介素（IL）‐10、干扰素（IFN）‐γ和干扰素诱导蛋白（IP）‐10抗体进行免疫组化。HIV阳性患者中有10人患有牛皮癣，14人患有瘙痒症，15人患有卡波西氏肉瘤。HIV阴性的对照组包括12名牛皮癣患者和2名KS患者。利用数字成像系统对每种疾病的四个代表性皮肤切片进行光密度分析，确认细胞因子染色的半定量分析。光密度分析采用方差分析和t检验。我们发现覆盖在HIV+卡波西肉瘤上的表皮是高增殖的，并且IP‐10、IFN‐γ和IL‐10的含量最高（P=0.0001）。TNF - α染色中HIV+瘙痒最高（P=0.0001）。HIV+牛皮癣代表所有四种细胞因子的中间状态。与病变相邻的正常皮肤显示相同的相对模式，强度较低。经常在艾滋病毒和免疫缺陷的情况下出现的皮肤病具有相对不同的细胞因子表达水平和模式，这可能反映免疫功能障碍、对艾滋病毒和机会性感染的反应。
AIDS is known to cause a shift of cytokines in the periphery. However, predominant cytokines in skin of patients with HIV‐associated skin diseases have not been clearly defined. We hypothesized that there are distinct cytokine profiles that distinguish among the different clinical manifestations of AIDS‐related skin diseases. To test this hypothesis, lesional and non‐lesional skin was biopsied from 53 HIV+patients with Kaposi's sarcoma (KS), psoriasis, and pruritus due to eosinophilic folliculitis, and from HIV negative controls with psoriasis or KS prior to therapy. Immunohistochemistry was performed with antibodies to tumor necrosis factor (TNF)‐α, interleukin (IL)‐10, interferon (IFN)‐γ, and interferon‐inducible protein (IP)‐10. HIV positive individuals included 10 with psoriasis, 14 with pruritus, and 15 with Kaposi's sarcoma. HIV negative controls included 12 with psoriasis and two with KS. Semi‐quantitative analysis of cytokine staining was confirmed by optical density using a digital imaging system on four representative skin sections from each disease. Optical density analyses were conducted using ANOVA andt‐tests. We found that epidermis overlying HIV+Kaposi's sarcoma was hyperproliferative and was highest in IP‐10, IFN‐γ, and IL‐10 (P=0.0001). HIV+pruritus was significantly highest in TNF‐α (P=0.0001) staining. HIV+psoriasis represented an intermediate state for all four cytokines. Normal skin adjacent to lesions showed the same relative patterns, with lower intensities. Skin diseases seen frequently in the setting of HIV and immunodeficiency have relatively distinct levels and patterns of cytokine expression that may reflect immune dysfunction, reactivity to HIV and to opportunistic infections.