Expression of Kruppel-like factor 5 in human gastric carcinomas
Expression of Kruppel-like factor 5 in human gastric carcinomas
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DOI：
10.1007/s00432-007-0265-2
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发表时间：
2008-02-01
影响因子：
3.6
通讯作者：
Yang, Han-Kwang
中科院分区：
文献类型：
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作者：
Kwak, Mi Kyung;Lee, Hyuk-Joon;Yang, Han-Kwang
目的Kruppel样因子5（KLF 5）是一种锌指转录因子，由于其在细胞生长、增殖、分化和肿瘤发生等多个系统中具有重要的调节作用而引起人们的关注。然而，其在人胃癌中的表达以前没有被描述。在这项研究中，我们研究了KLF 5的表达谱及其临床病理特征和表达之间的关系在胃癌carcinoma.Methods组织中获得的247例胃癌患者进行根治性胃切除术（R 0切除术）在外科，首尔国立大学医院从1995年1月至1995年6月，这些组织排列在组织阵列块。使用抗BTEB 2小鼠单克隆抗体（圣克鲁斯生物技术公司，结果KLF 5在45.7%（113/247）的肿瘤组织中表达。在早期胃癌（63.2vs.38.0%，p < 0.001）、无淋巴结转移胃癌（54.0vs.40.1%，p = 0.04）和<5cm胃癌（53.0vs.38.1%，p = 0.02）中，其表达率明显增高。KLF 5阳性表达者的5年生存率高于KLF 5阴性表达者（74.7%vs.62.2%，p = 0.057）。结论KLF 5在早期胃癌、小胃癌和无淋巴结转移的胃癌中表达率较高。通过单因素分析，发现其表达有利于手术后的生存。我们的研究首次描述了KLF 5在大量人类胃癌组织中的表达谱，并表明最近许多研究中显示的一致结果，即KLF 5表达减少发生在许多类型的人类肿瘤中。
Purpose Kruppel-like factor 5 (KLF5) is a zinc finger transcription factor, which has recently attracted attention because of its important regulatory activities linked to diverse functions such as cell growth, proliferation, differentiation, and tumorigenesis in a number of systems. However, its expression in human gastric cancer has not been described previously. In this study, we investigated the expression profile of KLF5 and the relationship between its clinicopathologic features and expression in gastric carcinomas.Methods Tissues were obtained from 247 gastric carcinoma patients who underwent curative gastrectomy (R0 resection) at the Department of Surgery, Seoul National University Hospital from January 1995 to June 1995, and these tissues were arranged in tissue array blocks. KLF5 expression was analyzed by immunohistochemical staining using anti-BTEB2 mouse monoclonal antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).Results Overall KLF5 was found to be expressed in 45.7% (113/247) of tumor tissues. Moreover, its expression rate was significantly high in early-staged gastric cancer (63.2 vs. 38.0%, p < 0.001), in gastric cancer without lymph node metastasis (54.0 vs. 40.1%, p = 0.04), and in tumors < 5 cm in size (53.0 vs. 38.1%, p = 0.02). The 5-year survival rate of patients with KLF5-positive tumors was higher than those of patients with KLF5-negative tumors, although this was not statistically significant (74.7 vs. 62.2%, p = 0.057).Conclusion KLF5 expression rate was high in early-staged gastric cancer, in small gastric cancer tissues and in gastric cancer without lymph node metastasis. By univariate analysis, its expression was found to favor survival after surgery. Our study describes for the first time the expression profile of KLF5 in a large number of human gastric cancer tissues and suggests consistent results shown in many recent studies that reduction of KLF5 expression occurs in many types of human tumor.