Cell surface characteristics of nontypeable isolates of Streptococcus suis
Cell surface characteristics of nontypeable isolates of Streptococcus suis
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DOI：
10.1111/j.1574-6968.2010.02086.x
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发表时间：
2010-10-01
影响因子：
2.1
通讯作者：
Grenier, Daniel
中科院分区：
文献类型：
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作者：
Bonifait, Laetitia;Gottschalk, Marcelo;Grenier, Daniel
猪链球菌是引起各种猪感染的世界性病原，也是人畜共患病的重要病原。猪链球菌根据其血清型进行分类，目前已识别35个血清型。这项研究的目的是根据非分型猪链球菌的细胞表面特性来确定它们的特征，并将它们与最常与感染相关的2型血清菌株进行比较。从感染动物中分离的7株非分型猪链球菌比2型血清分离株具有更强的附着在纤维连接蛋白包裹的聚苯乙烯表面的能力。还测试了三个非分型菌株与内皮细胞的黏附能力，发现与2型血清分离株相比，它们的黏附量更高。电子显微镜分析显示，在7个非分型分离株中没有被膜，这与细胞表面的疏水性比2型分离株高得多。所有非分型猪链球菌分离株也显示出形成生物膜的能力，而2型血清分离株不能形成生物被膜。总之，本研究中检测的非分型猪链球菌分离株具有不同于2型血清分离株的表面特性。
Streptococcus suis is a worldwide cause of various swine infections and is also an important agent of zoonosis. Strains of S. suis are classified according to their serotype, and currently, 35 serotypes are recognized. The aim of this study was to characterize nontypeable isolates of S. suis with regard to their cell surface properties and compare them with serotype 2 strains, the most frequently associated with infections. The seven nontypeable strains of S. suis isolated from infected animals demonstrated a stronger capacity to adhere to a fibronectin-coated polystyrene surface than the serotype 2 isolates. Three nontypeable strains were also tested for their ability to adhere to endothelial cells and were found to attach in higher amounts compared with the serotype 2 isolates. Electron microscopy analysis revealed the absence of a capsule in the seven nontypeable isolates, which correlated with a much higher cell surface hydrophobicity than that of serotype 2 isolates. All nontypeable isolates of S. suis also showed the capacity to form a biofilm while serotype 2 isolates were unable to do so. In conclusion, the nontypeable isolates of S. suis examined in this study possess surface properties different from those of serotype 2 isolates.