The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization
The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization
复制标题
DOI：
10.1111/j.1462-2920.2004.00710.x
复制
发表时间：
2005-02-01
影响因子：
5.1
通讯作者：
Witte, U
中科院分区：
文献类型：
--
作者：
Bühring, SI;Elvert, M;Witte, U
本研究描述了微生物群落结构的三个桑迪沉积物站，不同的中值粒径和渗透性在德国湾的北海南部。微生物群落进行了调查，脂质生物标志物分析和荧光原位杂交。为了进一步表征，我们确定了生物标志物的稳定碳同位素组成。所鉴定的生物标志物属于不同的细菌群，例如噬细胞-黄杆菌群和硫酸盐还原菌（SRB）的成员。为了支持这些发现，使用不同的荧光原位杂交探针进行了调查，专门针对噬细胞黄杆菌，γ-变形菌和SRB的不同成员。细菌脂肪酸的相对丰度的深度配置文件显示升高的细沉积物的次表层峰值，而在其他桑迪沉积物站的浓度随深度变化较小。虽然氧气渗透到更深的粗糙和更具渗透性的沉积物中，SRB生物标志物同样丰富，表明在这些环境中的缺氧生态位。我们在所有沉积物类型以及在表面和更深处检测到SRB，这表明SRB在含氧海洋沉积物中起着比以前认为的更重要的作用。
This study describes the microbial community structure of three sandy sediment stations that differed with respect to median grain size and permeability in the German Bight of the Southern North Sea. The microbial community was investigated using lipid biomarker analyses and fluorescence in situ hybridization. For further characterization we determined the stable carbon isotope composition of the biomarkers. Biomarkers identified belong to different bacterial groups such as members of the Cytophaga-Flavobacterium cluster and sulfate-reducing bacteria (SRB). To support these findings, investigations using different fluorescent in situ hybridization probes were performed, specifically targeting Cytophaga-Flavobacterium, gamma-Proteobacteria and different members of the SRB. Depth profiles of bacterial fatty acid relative abundances revealed elevated subsurface peaks for the fine sediment, whereas at the other sandy sediment stations the concentrations were less variable with depth. Although oxygen penetrates deeper into the coarser and more permeable sediments, the SRB biomarkers are similarly abundant, indicating suboxic to anoxic niches in these environments. We detected SRB in all sediment types as well as in the surface and at greater depth, which suggests that SRB play a more important role in oxygenated marine sediments than previously thought.