Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43
Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43
复制标题
DOI：
10.1006/mpat.1996.0024
复制
发表时间：
1996-05-01
影响因子：
3.8
通讯作者：
Peng, HL
中科院分区：
文献类型：
--
作者：
Chang, HY;Lee, JH;Peng, HL
通过转座子诱变产生肺炎克雷伯氏菌野生型菌株CG 43的非粘液样突变体。其中一个突变体不能发酵半乳糖，命名为CG 43 -17。观察到细菌表面的改变，包括荚膜、脂多糖和几种外膜蛋白。该突变株对小鼠无毒力，对人血清高度敏感。galETK操纵子不能弥补这些缺陷。CG 43 -17的UDP-葡萄糖焦磷酸化酶活性降低，表明它是一个galU突变体。这种可能性得到证实，因为亲本表型可以完全恢复的突变体，通过转化它与人肝cDNA编码UDP-葡萄糖焦磷酸化酶。(C)出版社：Academic Press Limited。
Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the galETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a galU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase. (C) 1996 Academic Press Limited.