Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype
Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype
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DOI：
10.1111/j.1365-2761.2006.00755.x
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发表时间：
2006-10-01
影响因子：
2.5
通讯作者：
Winton, J.
中科院分区：
文献类型：
--
作者：
Elsayed, E.;Faisal, M.;Winton, J.
病毒性出血性败血症病毒（VHSV）是从2003年从美国密歇根州圣克莱尔湖西北部捕获的麝香犬Esox masquinongy（Mitchill）中分离出来的。受影响的鱼表现出充血的内部器官，鱼鳔的内壁增厚，并含有许多出芽，充满液体的囊泡。用感染鱼的肾和脾组织匀浆接种鱼细胞系分离病毒。早在接种后24 h就出现细胞变圆和颗粒的局灶性区域，并迅速扩大，到96 h时破坏整个细胞片层。电子显微镜检查显示病毒粒子长170-180 nm，宽60-70 nm，具有弹状病毒典型的子弹形形态。经逆转录-聚合酶链反应证实为VHSV。整个核蛋白和糖蛋白基因的序列分析显示，该病毒是北美VHSV基因型的成员;然而，该分离株足够独特，可以被认为是一个单独的亚系，这表明它的起源可能来自居住在美国或加拿大东部沿海地区的海洋物种。
Viral haemorrhagic septicaemia virus (VHSV) was isolated from muskellunge, Esox masquinongy (Mitchill), caught from the NW portion of Lake St Clair, Michigan, USA in 2003. Affected fish exhibited congestion of internal organs; the inner wall of the swim bladder was thickened and contained numerous budding, fluid-filled vesicles. A virus was isolated using fish cell lines inoculated with a homogenate of kidney and spleen tissues from affected fish. Focal areas of cell rounding and granulation appeared as early as 24 h post-inoculation and expanded rapidly to destroy the entire cell sheet by 96 h. Electron microscopy revealed virions that were 170-180 nm in length by 60-70 nm in width having a bullet-shaped morphology typical of rhabdoviruses. The virus was confirmed as VHSV by reverse transcriptase-polymerase chain reaction. Sequence analysis of the entire nucleoprotein and glycoprotein genes revealed the virus was a member of the North American genotype of VHSV; however, the isolate was sufficiently distinct to be considered a separate sublineage, suggesting its origin may have been from marine species inhabiting the eastern coastal areas of the USA or Canada.