Preparation of primary cultured dopaminergic neurons from mouse brain.
Preparation of primary cultured dopaminergic neurons from mouse brain.
复制标题
DOI：
10.1007/978-1-62703-444-9_6
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Xia, Zhengui
中科院分区：
文献类型：
--
作者：
Choi, Won-Seok;Kim, Hyung-Wook;Xia, Zhengui
多巴胺能神经元参与多种正常的脑功能，这些神经元的变性导致人类疾病。研究多巴胺能神经元如何响应细胞外信号和调节多巴胺能神经元存活和死亡的分子机制通常需要可靠的、可重复的和高质量的多巴胺能神经元原代培养物。本文介绍了小鼠胚胎中脑神经元的分离培养方法。我们利用Aclar膜制成的盖玻片来最大化培养中存活的多巴胺能神经元的数量，并利用酪氨酸羟化酶（TH）的免疫细胞化学来鉴定多巴胺能神经元。
Dopaminergic neurons are involved in a variety of normal brain functions; degenerations of these neurons cause diseases in human. Investigation of how dopaminergic neurons respond to extracellular signals and molecular mechanisms regulating dopaminergic neuron survival and death often requires reliable, reproducible, and high-quality primary cultures of dopaminergic neurons. Here, we described methods to dissect and culture these neurons from embryonic mesencephalon of mouse brain. We utilize coverslips made from Aclar film to maximize the number of surviving dopaminergic neuron in the culture and immunocytochemistry of tyrosine hydroxylase (TH) to identify dopaminergic neuron.