Toll-like receptor 9 expression is not required for CpG DNA-aided cross-presentation of DNA-conjugated antigens but essential for cross-priming of CD8 T cells
Toll-like receptor 9 expression is not required for CpG DNA-aided cross-presentation of DNA-conjugated antigens but essential for cross-priming of CD8 T cells
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DOI：
10.4049/jimmunol.170.6.2802
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发表时间：
2003-03-15
影响因子：
4.4
通讯作者：
Wagner, H
中科院分区：
文献类型：
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作者：
Heit, A;Maurer, T;Wagner, H
免疫刺激性CpG DNA与OVA的共价连接导致CpG DNA辅助的树突状细胞（DC）对OVA的交叉呈递。在体内，交叉呈递对于OVA特异性CD 8 T细胞的交叉致敏是有条件的。在这项研究中，我们研究了CpG DNA受体Toll样受体（TLR）9在CpG DNA辅助交叉呈递和交叉启动中的参与。尽管CpG DNA辅助的交叉呈递在TLR 9缺陷细胞中没有改变，但TLR 9是APC成熟所必需的，允许交叉引发，从而导致CTL功能。这些发现暗示TLR 9不触发CpG-OVA缀合物的内吞作用，但激活内吞作用下游的DC
Covalent linkage of immunostimulatory CpG DNA to OVA results in CpG DNA-aided cross-presentation of OVA by dendritic cells (DCs). In vivo, cross-presentation is conditional for cross-priming of OVA-specific CD8 T cells. In this study, we investigated the involvement of the CpG DNA receptor Toll-like receptor (TLR)9 in CpG DNA-aided cross-presentation and cross-priming. Although CpG DNA-aided cross-presentation is not altered in TLR9-deficient cells, TLR9 is required for maturation of APC allowing cross-priming, as resulting in CTL function. These findings imply that TLR9 does not trigger endocytosis of CpG-OVA conjugates, but activates DCs downstream of endocytosis