Communication between paired chondrocytes in the superficial zone of articular cartilage
Communication between paired chondrocytes in the superficial zone of articular cartilage
复制标题
DOI：
10.1111/j.0021-8782.2004.00350.x
复制
发表时间：
2004-11-01
影响因子：
2.4
通讯作者：
Matyas, JR
中科院分区：
文献类型：
--
作者：
Chi, SS;Rattner, JB;Matyas, JR
损伤或疾病损伤的软骨的再生和修复是骨科科学的主要目标，取决于对细胞外基质和软骨细胞的结构和功能的理解。在这项研究中，我们探讨了在原位组织和软骨细胞之间的潜在相互作用在成年兔关节软骨浅层。在该区域中观察到一些软骨细胞紧密地在一起并且似乎是成对的，而其他软骨细胞是孤立的。软骨细胞对的共享表面被细胞外基质的窄板分开，从两个细胞延伸到小的细胞质突起。此外，主要的细胞标志物，如细胞核和中心体以及一些细胞内蛋白质，如连接蛋白-43的空间分布，往往反映了这个矩阵板。光漂白后的荧光恢复显示荧光染料钙黄绿素-AM染料可以在成对细胞之间通过，并且该染料的通过可以被差距连接阻断剂辛醇抑制。这些结果表明，在成人关节软骨的浅层细胞之间的快速细胞通讯是可能的，这挑战了目前认为这些软骨细胞在隔离功能。
The regeneration and repair of cartilage damaged by injury or disease, a major goal of orthopaedic science, depends on understanding the structure and function of both the extracellular matrix and the chondrocytes. In this study, we explored the in situ organization and potential interactions between chondrocytes in the superficial zone of adult rabbit articular cartilage. Some chondrocytes in this zone were observed close together and appeared to be paired whereas others were solitary. The shared surfaces of a chondrocyte pair were separated by a narrow plate of extracellular matrix, into which extended small cytoplasmic projections from both cells. Furthermore, the spatial distribution of major cellular landmarks, such as the nucleus and centrosome as well as some intracellular proteins such as connexin-43, tended to be mirrored about this matrix plate. Fluorescence recovery after photobleaching revealed the fluorescent dye calcein-AM dye can pass between paired cells, and that the passage of this dye can be inhibited by the gap junction blocker octanol. These results illustrate that rapid cellular communication is possible between cells in the superficial layer of adult articular cartilage, which challenges the current thinking that these chondrocytes function in isolation.