Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal
Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal
复制标题
DOI：
10.1007/978-1-59745-280-9_4
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Kornblum, Harley I.
中科院分区：
文献类型：
--
作者：
Nakano, Ichiro;Kornblum, Harley I.
神经干细胞（Neural stein cells， NSC）具有自我更新和多能性，可产生神经元和胶质细胞。最近的研究表明，脑肿瘤（BT）含有像NSC一样自我更新和多能的细胞，产生脑肿瘤中发现的不同类型的细胞。这些脑肿瘤干细胞是一种肿瘤干细胞，在实验动物体内能够形成与母体肿瘤相转移的肿瘤。我们实验室和其他实验室的研究表明，脑肿瘤干细胞和NSC具有相似的增殖机制和途径。例如，我们已经发现AMPK/snf1激酶之一，母体胚胎亮氨酸拉链激酶（MELK），在NSC和恶性脑肿瘤以及脑肿瘤干细胞富集的细胞培养中高度表达。对转基因M elk报告基因小鼠的分析表明，MELK在体内的NSC中表达，我们的体外研究表明，MELK是NSC自我更新所必需的。我们还发现MELK对于假定的BT干细胞的增殖是必需的。本章以MELK干细胞为例，介绍了NSC和BT干细胞的培养方法，并分析了这些细胞自我更新的调控途径。
Neural stein cells (NSC) self-renew and arc multipotent, producing neurons and glia. Recent studies have shown that brain tumors (BT) contain cells that, like NSC, self-renew and are multipotent, producing the different types of cells found within the brain tumors. These brain tumor stem cells are a kind of cancer stem cell, competent to form tumors that manic the parent tumor in experimental animals. Studies from our laboratory and others have demonstrated that brain tumor stem cells and NSC share similar mechanisms and pathways for proliferation. For example, we have identified that one of the AMPK/snf1 kinases, maternal embryonic leucine zipper kinase (MELK), is highly expressed in NSC and malignant brain tumors, as well as in brain tumor stem cell-enriched cell cultures. Analysis of transgenic M ELK-reporter mice indicated that MELK is expressed in NSC in vivo, and our in vitro studies demonstrated that MELK is required for NSC self-renewal. We have also found that MELK is required for proliferation of putative BT stem cells. Utilizing our studies with MELK as an example, this chapter describes methods to culture NSC and BT stem cells, and to analyze the pathways, which regulate self-renewal of those cells.