Isolation of Murine Skin Resident and Migratory Dendritic Cells via Enzymatic Digestion.
Isolation of Murine Skin Resident and Migratory Dendritic Cells via Enzymatic Digestion.
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DOI：
10.1002/cpim.45
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发表时间：
2018-04-01
影响因子：
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通讯作者：
Kaplan, Daniel H
中科院分区：
文献类型：
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作者：
Kashem, Sakeen W;Kaplan, Daniel H
树突状细胞(DC)是专业抗原提呈细胞(APC)的高度特化亚群，存在于外周组织和淋巴组织中。DC在外周捕获抗原，并迁移到淋巴结处，在那里它们激活初始T细胞。此外，树突状细胞最近被认为在组织内的先天性免疫中具有功能。在皮肤中，不同种类的树突状细胞存在于表皮和真皮内。皮肤DC亚群的分析由于不同的酶消化方案的要求而变得复杂，这些要求是从皮肤的不同解剖间隔中分离出APC。在这里，描述了从小鼠的表皮、真皮和皮肤引流淋巴结中分离DC的具体方法。2018年John Wiley&Sons，Inc.
Dendritic cells (DCs) are a highly specialized subset of professional antigen-presenting cells (APCs) that reside in peripheral and lymphoid tissues. DCs capture antigen in the periphery and migrate to the lymph node where they prime naive T cells. In addition, DCs have been recently appreciated to have function in innate immunity within tissues. In the skin, heterogeneous populations of DCs reside within the epidermis and the dermis. Analysis of the cutaneous DC subsets is complicated by requirements of distinct enzymatic digestion protocols for isolation of APCs from distinct anatomical compartments of the skin. Here, specific approaches for isolation of DCs from the epidermis, dermis, and the skin-draining lymph nodes of mice are described. © 2018 by John Wiley & Sons, Inc.