Highly rapid and diverse sex chromosome evolution in the Odorrana frog species complex
Highly rapid and diverse sex chromosome evolution in the Odorrana frog species complex
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DOI：
10.1111/dgd.12800
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发表时间：
2022-08-25
影响因子：
2.5
通讯作者：
Miura，Ikuo
中科院分区：
文献类型：
--
作者：
Katsumi，Taito;Shams，Foyez;Miura，Ikuo
变温脊椎动物的性染色体的特点是在物种或种群水平上快速且多样化的进化。我们之前的研究表明，台湾青蛙 Odorrana swinhoana（2n = 26）具有独特的多性染色体系统，该系统是由染色体 1、3 和 7 之间的三个连续易位产生的。为了揭示 Odorrana 物种复合体中性染色体的进化史，我们首先在 O 祖先型群体中鉴定了易位发生之前的原始同态性染色体。斯温霍阿纳。然后，我们将调查范围扩大到分布在两个小岛上的一种密切相关的日本物种 Odorrana utsomiyaorum。我们使用高通量核基因组方法来分析单核苷酸多态性并鉴定性别连锁标记。那些与theO隔离的人。 swinhoana祖先型群体被发现与1号染色体对齐并表现出雄性异配性。相比之下，几乎所有的性别连锁标记都从 O 中分离出来。宇都宫鲁姆在女性中是杂合的，在男性中是纯合的，并且与9号染色体对齐。在形态学上，我们证实9号染色体在女性中是异形的，显示出ZZ-ZW性别决定系统，其中W染色体沿染色体轴以条纹图案异染色质。这些结果表明，在两个物种分化后，祖先同态性染色体1经历了高度快速和多样化的进化，即O中两个常染色体的顺序易位。 swinhoana，并转变为 O 中的 9 号染色体。 utsomiyaorum，从 XY 异配型过渡到 ZW 异配型并转变为异型。
Sex chromosomes in poikilothermal vertebrates are characterized by rapid and diverse evolution at the species or population level. Our previous study revealed that the Taiwanese frogOdorrana swinhoana(2n = 26) has a unique system of multiple sex chromosomes created by three sequential translocations among chromosomes 1, 3, and 7. To reveal the evolutionary history of sex chromosomes in theOdorranaspecies complex, we first identified the original, homomorphic sex chromosomes, prior to the occurrence of translocations, in the ancestral‐type population ofO. swinhoana. Then, we extended the investigation to a closely related Japanese species,Odorrana utsunomiyaorum, which is distributed on two small islands. We used a high‐throughput nuclear genomic approach to analyze single‐nucleotide polymorphisms and identify the sex‐linked markers. Those isolated from theO. swinhoanaancestral‐type population were found to be aligned to chromosome 1 and showed male heterogamety. In contrast, almost all the sex‐linked markers isolated fromO. utsunomiyaorumwere heterozygous in females and homozygous in males and were aligned to chromosome 9. Morphologically, we confirmed chromosome 9 to be heteromorphic in females, showing a ZZ‐ZW sex determination system, in which the W chromosomes were heterochromatinized in a stripe pattern along the chromosome axis. These results indicated that after divergence of the two species, the ancestral homomorphic sex chromosome 1 underwent highly rapid and diverse evolution, i.e., sequential translocations with two autosomes inO. swinhoana,and turnover to chromosome 9 inO. utsunomiyaorum, with a transition from XY to ZW heterogamety and change to heteromorphy.