retro-Tango enables versatile retrograde circuit tracing in Drosophila.
retro-Tango enables versatile retrograde circuit tracing in Drosophila.
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DOI：
10.7554/elife.85041
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发表时间：
2023-05-11
期刊：
影响因子：
7.7
通讯作者：
Barnea G
中科院分区：
文献类型：
--
作者：
Sorkaç A;Moșneanu RA;Crown AM;Savaş D;Okoro AM;Memiş E;Talay M;Barnea G
跨突触追踪方法是研究神经回路的重要工具。尽管在果蝇中已经开发了几种顺行追踪方法和一种有针对性的逆行追踪工具，但仍然需要一个公正、用户友好和灵活的逆行追踪系统。在这里，我们描述了Rettro-Tango，一种在果蝇中进行跨突触、逆行电路追踪和操作的方法。在这个基因编码的系统中，突触的配体-受体相互作用触发了细胞内的信号级联，导致突触前神经元中报告基因的表达。重要的是，级联元素的泛神经元表达使这种方法变得多才多艺，使其不仅能够测试假设，还能够产生假设。我们在不同的电路中验证了Rettro-Tango，并将我们的发现与果蝇半脑的电子显微镜重建进行了比较，并对其进行了基准测试。我们的实验确立了Rettro-Tango作为神经科学研究中电路追踪的关键方法。
Transsynaptic tracing methods are crucial tools in studying neural circuits. Although a couple of anterograde tracing methods and a targeted retrograde tool have been developed in Drosophila melanogaster, there is still need for an unbiased, user-friendly, and flexible retrograde tracing system. Here, we describe retro-Tango, a method for transsynaptic, retrograde circuit tracing and manipulation in Drosophila. In this genetically encoded system, a ligand-receptor interaction at the synapse triggers an intracellular signaling cascade that results in reporter gene expression in presynaptic neurons. Importantly, panneuronal expression of the elements of the cascade renders this method versatile, enabling its use not only to test hypotheses but also to generate them. We validate retro-Tango in various circuits and benchmark it by comparing our findings with the electron microscopy reconstruction of the Drosophila hemibrain. Our experiments establish retro-Tango as a key method for circuit tracing in neuroscience research.