Secondary cytotoxic allograft responses in vitro. II. Differentiation Of memory T Cells into cytotoxic T lymphocytes in the absence of cell proliferation
Secondary cytotoxic allograft responses in vitro. II. Differentiation Of memory T Cells into cytotoxic T lymphocytes in the absence of cell proliferation
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体外二次细胞毒性同种异体移植物反应。
DOI：
10.1002/eji.1830060105
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发表时间：
1976
影响因子：
5.4
通讯作者：
M. Röllinghoff
中科院分区：
文献类型：
--
作者：
H. Wagner;M. Röllinghoff
在“单向”混合淋巴细胞培养物中诱导小鼠细胞毒性 T 淋巴细胞 (CTL)，并通过 1 × g 速度沉降研究其物理特性。 CTL祖细胞在体外分化为初级和次级CTL与应答细胞大小的特征性变化是平行的。富含原代母细胞 CTL 的分级细胞恢复为克隆限制的“非溶解性”次级 T 淋巴细胞。再次接触抗原后，这些淋巴细胞在 18-32 小时内分化为次级 CTL。这是在没有细胞增殖的情况下发生的，并且可以由紫外线照射的同种异体刺激细胞触发。有人认为，诱导初级或次级细胞毒性 T 细胞反应的不同特征反映了未引发的 T 细胞和记忆 T 淋巴细胞之间的质量差异。
Murine cytotoxic T lymphocytes (CTL) were induced in “one‐way” mixed lymphocyte cultures and their physical characteristics investigated by velocity sedimentation at 1 × g. The in vitro differentiation of progenitors of CTL into primary and secondary CTL was paralleled by characteristic changes in the size of the responder cells. Fractionated cells enriched for primary blast CTL reverted into clonally restricted “nonlytic” secondary T lymphocytes. Upon antigenic reexposure, these lymphocytes differentiated into secondary CTL within 18–32 h. This took place in the absence of cell proliferation and could be triggered by UV light irradiated allogeneic stimulator cells. It is suggested that the different characteristics for the induction of either a primary or secondary cytotoxic T cell response reflect qualitative differences between unprimed T cells and memory T lymphocytes.