In vivo gene transfer into muscle via electro-sonoporation.
In vivo gene transfer into muscle via electro-sonoporation.
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通过电声穿孔将体内基因转移到肌肉中。
DOI：
10.1089/10430340260395929
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发表时间：
2002
影响因子：
4.2
通讯作者：
K. Sugimachi
中科院分区：
文献类型：
--
作者：
Y. Yamashita;M. Shimada;K. Tachibana;N. Harimoto;E. Tsujita;K. Shirabe;J. Miyazaki;K. Sugimachi
在体内基因转移的非病毒技术中，电穿孔是一种简单、有效、廉价和安全的技术。为了上调转移基因的表达水平，我们研究了体内电声穿孔的适用性，该方法由电脉冲和超声波组合组成，用于利用编码荧光素酶和小鼠白细胞介素-12 （mIL-12）的质粒DNA进行基因转移。用质粒DNA注射小鼠股四头肌，超声穿孔5分钟，在超声穿孔持续时间中间用一对电极板进行电穿孔。三天后，与单独进行电穿孔的小鼠相比，接受电声穿孔的小鼠显示出高两倍的荧光素酶活性和低的股四头肌组织损伤。基因转移后，经电声穿孔小鼠血清mIL-12水平（峰值为25.5 ng/ml）比单独经电穿孔小鼠血清mIL-12水平（峰值为14.3 ng/ml）高两倍，并在基因转移后28天维持13.9 ng/ml的高血清水平。与白蛋白微泡超声造影剂相比，质粒DNA溶液NaCl浓度为0.85%时，电声穿孔基因转移效果更好。这些结果表明，通过电声穿孔将基因转移到肌肉中可以为基因在体内转移提供一种新的有效的非病毒技术。
Among the nonviral techniques for gene transfer in vivo, electroporation is simple, potent, inexpensive, and safe. To upregulate the expression levels of the transferred gene, we investigated the applicability of in vivo electro-sonoporation, which consists of a combination of electric pulse and ultrasound, for gene transfer using plasmid DNA encoding luciferase and mouse interleukin-12 (mIL-12). The quadriceps muscles of mice were injected with plasmid DNA, then sonoporated for 5 min, and electroporated by a pair of electrode plates at the middle of the duration of sonoporation. Three days later, mice that had undergone electro-sonoporation demonstrated twofold higher luciferase activity and low tissue damage in quadriceps muscle compared to mice having undergone electroporation alone. Serum mIL-12 levels in mice that had undergone electro-sonoporation (peaking at 25.5 ng/ml) were twofold higher after gene transfer than were those in mice having undergone electroporation alone (peaking at 14.3 ng/ml), and maintained high serum level of 13.9 ng/ml at 28 days after gene transfer. The efficacy of gene transfer via electro-sonoporation was superior when the plasmid DNA solution was 0.85% NaCl compared to albumin microbubble echo-contrast material. These results demonstrated that gene transfer into muscle via electro-sonoporation could provide a new potent nonviral technique for gene transfer in vivo.
影响因子：
2.9
作者：
Huntington Potter
通讯作者：
Huntington Potter
DOI：
10.1073/pnas.84.23.8463
发表时间：
1987-12-01
影响因子：
11.1
作者：
FECHHEIMER, M;BOYLAN, JF;ZIMMER, SG
通讯作者：
ZIMMER, SG
通过碎石机冲击波体内转染黑色素瘤细胞。
DOI：
--
发表时间：
1998
期刊：
Cancer research.
影响因子：
--
作者：
Bao,S;Thrall,BD;Gies,RA;Miller,DL
通讯作者：
Miller,DL