Purification and some properties of ATP-citrate lyase from rat brain.
Purification and some properties of ATP-citrate lyase from rat brain.
复制标题
大鼠脑中 ATP-柠檬酸裂解酶的纯化及其一些特性。
DOI：
10.1016/0003-9861(83)90133-9
复制
发表时间：
1983
影响因子：
3.9
通讯作者：
Srere,PA
中科院分区：
文献类型：
--
作者：
Szutowicz,A;Srere,PA
ATP-citrate lyase has been purified from rat brain by a new procedure which yields an enzyme of specific activity of 21 U/mg protein (37 °C) (2050-fold purification). Purity (by sodium dodecyl sulfate-gel electrophoresis) of the preparation was comparable to that of rat liver ATP-citrate lyase of similar specific activity. Both brain and liver ATP-citrate lyase have the same electrophoretic mobility, as well as the same immunoreactivity against specific rabbit anti-rat liver ATP-citrate lyase antibody. These data indicate that rat brain ATP-citrate lyase is similar or identical to that present in rat liver. Intraperitoneally injected32Piwas incorporated into the structural phosphate of ATP-citrate lyase in rat liver but not into the rat brain enzyme.
