Expression analysis of facilitative glucose transporters (GLUTs) in human thyroid carcinoma cell lines and primary tumors
Expression analysis of facilitative glucose transporters (GLUTs) in human thyroid carcinoma cell lines and primary tumors
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DOI：
10.1016/j.mce.2008.05.003
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发表时间：
2008-09-10
影响因子：
4.1
通讯作者：
Elisei, Rossella
中科院分区：
文献类型：
--
作者：
Ciampi, Raffaele;Vivaldi, Agnese;Elisei, Rossella
氟-18-氟-2-脱氧-D-葡萄糖正电子发射断层扫描(FDG-PET)是基于细胞摄取葡萄糖的能力。虽然葡萄糖转运蛋白GLUT1在甲状腺肿瘤中的表达显著增加，但有关其他GLUT亚型表达的数据很少。我们研究了来自间变性(ARO，Fro)、乳头状癌(NPA)、滤泡性(WRO)和髓样(TT)人甲状腺癌的几种GLUT亚型的表达。同时检测了157例甲状腺良恶性病变组织中Glut1和Gluts的表达。实时荧光定量RT-PCR分析显示，低分化细胞(ARO)中GLUT1基因的表达水平较高。FRO)，而GLUT3mRNA水平在高分化细胞(NPA、WRO)中普遍存在。相应地，Western印迹显示GLUT1蛋白在ARO和FRO中高表达并正确定位于膜，GLUT3蛋白在NPA和WRO中高表达和正确的膜靶向。所有细胞系均能摄取不同速率的H-3-脱氧葡萄糖。对人甲状腺组织中GLUT1和GLUT3mRNA表达的分析表明，与正常组织相比，恶性甲状腺组织中GLUT1的表达普遍，但GLUT3的表达不明显。结论：GLUT1和GLUT3是甲状腺肿瘤细胞中最重要的葡萄糖转运蛋白。特别是，GLUT1在低分化细胞(ARO和FRO)中最常见，而GLUT3在高分化细胞(NPA和WRO)中最常见。在人类甲状腺肿瘤中发现了类似的GLUT1而不是GLUT3的表达模式。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Fluorine-18-fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) is based on cell capability to take-up glucose. While a significantly higher expression of the glucose transporter GLUT1 has been reported in thyroid tumors only few data are available on the expression of other GLUT isoforms.We studied several GLUT isoforms expression in thyroid tumor cell lines deriving from anaplastic (ARO, FRO), papillary (NPA), follicular (WRO) and medullary (TT) human thyroid carcinoma. GLUT1 and GLUTS were also studied in 157 human thyroid malignant and benign tissues.Quantitative Real-time RT-PCR analysis revealed that GLUT1 mRNA levels were higher in less-differentiated cells (ARO. FRO) while GLUT3 mRNA levels were prevalent in well-differentiated cells (NPA, WRO). Accordingly, Western blot showed high expression and correct membrane targeting of GLUT1 protein in ARO and FRO and of GLUT3 protein in NPA and WRO. All cell lines were able to take-up different rates of H-3-deoxy-glucose. The analysis of GLUT1 and GLUT3 mRNA expression in human thyroid tissues showed the prevalence of GLUT1, but not of GLUT3, in malignant with respect to normal tissues. Finally, both GLUT1 and GLUT3 showed a slightly higher expression in anaplastic than in well-differentiated tumors.In conclusion, we showed that GLUT1 and GLUT3 were the most important glucose transporters in the thyroid tumoral cells. In particular GLUT1 was the most prevalent in less-differentiated cells (ARO and FRO) while GLUT3 was the most prevalent in well-differentiated cells (NPA and WRO). A similar pattern of expression was found for GLUT1 but not for GLUT3 in human thyroid tumors. (c) 2008 Elsevier Ireland Ltd. All rights reserved.