Modulations of the thylakoid system in snow xanthophycean alga cultured in the dark for two months: comparison between microspectrofluorimetric responses and morphological aspects
Modulations of the thylakoid system in snow xanthophycean alga cultured in the dark for two months: comparison between microspectrofluorimetric responses and morphological aspects
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DOI：
10.1007/s00709-005-0127-1
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发表时间：
2005-12-01
期刊：
影响因子：
2.9
通讯作者：
Pancaldi, S
中科院分区：
文献类型：
--
作者：
Baldisserotto, C;Ferroni, L;Pancaldi, S
研究人员在黑暗中培育两个月的雪丝状黄藻（Xantonema sp.）中研究了质体的反应，特别强调类囊体的结构和功能。对单个活细胞进行显微分光荧光分析，以研究光系统 II 叶绿素-蛋白质复合物组装的变化，并与光下生长的细胞进行比较。同时，监测了微观和亚微观质体形态以及光合色素含量的变化。在整个实验过程中，藻类中类囊体的层状结构保存得相对较好，而光系统II则因长时间的黑暗而发生分解和降解。相反，光系统 II 的光捕获复合体被证明在黑暗中长时间相对稳定。此外，暗示了外围触角在确定黄藻类藻类类囊体排列中的作用。尽管在 Xantonema sp. 中观察到的反应。可以从适应黑暗的角度来考虑，光系统 II 的光捕获复合体的逐渐不稳定证明了细胞在 35 天后开始老化。
The response of the plastid was studied, with a special emphasis on thylakoid structure and function, in a snow filamentous xanthophycean alga (Xanthonema sp.) incubated in darkness for two months. Microspectrofluorimetric analyses were performed on single living cells to study the variations in the assembly of the chlorophyll-protein complexes of photosystem II, in comparison with cells grown in light. In parallel, changes in micro- and submicroscopic plastid morphology and in photosynthetic pigment content were monitored. Throughout the experiment, the lamellar architecture of thylakoids in the alga was relatively well preserved, whereas photosystem II underwent disassembly and degradation triggered by prolonged darkness. Conversely, the light-harvesting complex of photosystem II proved to be relatively stable for long periods in darkness. Moreover, a role of the peripheral antennae in determining thylakoid arrangement in xanthophycean algae is implied. Although the responses observed in Xanthonema sp. can be considered in terms of acclimation to darkness, the progressive destabilisation of the light-harvesting complex of photosystem II testifies to incipient ageing of the cells after 35 days.