cpsA regulates mycotoxin production, morphogenesis and cell wall biosynthesis in the fungus Aspergillus nidulans.
cpsA regulates mycotoxin production, morphogenesis and cell wall biosynthesis in the fungus Aspergillus nidulans.
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DOI：
10.1111/mmi.13682
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发表时间：
2017-07
影响因子：
3.6
通讯作者：
Calvo AM
中科院分区：
文献类型：
--
作者：
Feng X;Ramamoorthy V;Pandit SS;Prieto A;Espeso EA;Calvo AM
模式真菌尼杜拉曲霉能合成包括杂色曲霉毒素(ST)在内的大量次生代谢物。这种毒素的生产受到全球监管机构VEA的积极控制。在没有VEA(ΔVEA)的情况下，ST的生物合成被阻止。之前，我们在ΔVEA菌株中进行了随机突变，并鉴定了能够合成ST的突变株，其中包括Rm1。RM1与基因组文库的互补显示，突变发生在一个被命名为CPSA的基因中。而在ΔVEA的遗传背景中，CpSA的缺失恢复了ST的产生，而在VEA的野生型背景中，CpSA的缺失减少并延迟了ST的生物合成，降低了ST基因的表达。此外，CPSA也是产生包括青霉素在内的影响PN基因表达的其他次生代谢物所必需的。此外，CPSA对于正常的无性和性发育是必要的。化学和显微镜分析表明，CPSA存在于细胞质小泡中，是正常细胞壁组成和完整性所必需的，影响黏附能力和氧化应激敏感性。CPSA在子囊菌中的保守性表明，CPSA同源物可能在其他真菌物种中具有类似的作用。
The model fungus Aspergillus nidulans synthesizes numerous secondary metabolites, including sterigmatocystin (ST). The production of this toxin is positively controlled by the global regulator veA. In the absence of veA (ΔveA), ST biosynthesis is blocked. Previously we performed random mutagenesis in a ΔveA strain and identified revertant mutants able to synthesize ST, among them RM1. Complementation of RM1 with a genomic library revealed that the mutation occurred in a gene designated as cpsA. While in the ΔveA genetic background cpsA deletion restores ST production, in a veA wild-type background absence of cpsA reduces and delays ST biosynthesis decreasing the expression of ST genes. Furthermore, cpsA is also necessary for the production of other secondary metabolites, including penicillin, affecting the expression of PN genes. In addition, cpsA is necessary for normal asexual and sexual development. Chemical and microscopy analyses revealed that CpsA is found in cytoplasmic vesicles and it is required for normal cell wall composition and integrity, affecting adhesion capacity and oxidative stress sensitivity. The conservation of cpsA in Ascomycetes suggests that cpsA homologs might have similar roles in other fungal species.