Specificity of regulatory CD4+CD25+ T cells for self-T cell receptor determinants
Specificity of regulatory CD4+CD25+ T cells for self-T cell receptor determinants
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DOI：
10.1002/jnr.20066
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发表时间：
2004-04-01
影响因子：
4.2
通讯作者：
Vandenbark, AA
中科院分区：
文献类型：
--
作者：
Buenafe, AC;Tsaknaridis, L;Vandenbark, AA
虽然CD 4（+）CD 25（+）T细胞谱系（Treg细胞）的表型和调节特性已得到很好的描述，但其特异性在很大程度上仍然未知。我们在此证明，CD 4（+）CD 25（+）Treg群体包括对生殖系V基因库中发现的广谱人类TCR CDR 2决定簇的识别，以及对重组可溶性T细胞受体（TCR）蛋白中存在的供体型非生殖系编码的CDR 3 β序列的识别。在对TCR有反应的T细胞系中证明了调节活性，但在对对照抗原有反应的T细胞系中没有。TCR反应性T细胞的抑制活性需要细胞-细胞接触，并且涉及CTLA-4 GITR、IL-10和IL-17。因此，T-T调节网络包括具有针对自身TCR决定簇的特异性的Treg细胞。(C)2004 Wiley-Liss，Inc.
Although the phenotypic and regulatory properties of the CD4(+)CD25(+) T cell lineage (Treg cells) have been well described, the specificities remain largely unknown. We demonstrate here that the CD4(+)CD25(+) Treg population includes the recognition of a broad spectrum of human TCR CDR2 determinants found in the germline V gene repertoire as well as that of a donotypic nongermline-encoded CDR3beta sequence present in a recombinant soluble T cell receptor (TCR) protein. Regulatory activity was demonstrated in T cell lines responsive to TCR but not in T cell lines responsive to control antigens. Inhibiltory activity of TCR-reactive T cells required cell-cell contact and involved CTLA-4 GITR, IL-10, and IL-17. Thus, the T-T regulatory network includes Treg cells with specificity directed toward self-TCR determinants. (C) 2004 Wiley-Liss, Inc.