SEUSS integrates transcriptional and epigenetic control of root stem cell organizer specification.
SEUSS integrates transcriptional and epigenetic control of root stem cell organizer specification.
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SEUSS 整合了根干细胞组织者规范的转录和表观遗传控制
DOI：
10.15252/embj.2020105047
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发表时间：
2020-10-15
期刊：
影响因子：
--
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Zhai H;Zhang X;You Y;Lin L;Zhou W;Li C
同源异型基因表达的适当调控对植物和动物干细胞的命运至关重要。在拟南芥中，WUSCHEL（WUS）相关的HOMEOBOX 5（WOX5）基因在一组称为静止中心（QC）的根干细胞组织者细胞中特异性表达，并在QC规范中发挥核心作用。在这里，我们报告说，SEUSS（SEU）蛋白，同源的动物LIM域结合（LDB）蛋白，组装一个功能性转录复合物，调节WOX5的表达和QC规范。SEU被主转录因子SCARECROW物理募集到WOX5启动子。随后，SEU将SET结构域甲基转移酶SDG 4物理募集到WOX5启动子，从而激活WOX5表达。因此，与其动物对应物类似，SEU作为多接头蛋白，将遗传和表观遗传调节因子的作用整合到协调的转录程序中，以控制根干细胞组织者的特化。转录衔接蛋白SEUSS通过募集组蛋白甲基转移酶SDG4促进拟南芥静止中心决定簇WOX5的表达。
Proper regulation of homeotic gene expression is critical for stem cell fate in both plants and animals. In Arabidopsis thaliana, the WUSCHEL (WUS)‐RELATED HOMEOBOX 5 (WOX5) gene is specifically expressed in a group of root stem cell organizer cells called the quiescent center (QC) and plays a central role in QC specification. Here, we report that the SEUSS (SEU) protein, homologous to the animal LIM‐domain binding (LDB) proteins, assembles a functional transcriptional complex that regulates WOX5 expression and QC specification. SEU is physically recruited to the WOX5 promoter by the master transcription factor SCARECROW. Subsequently, SEU physically recruits the SET domain methyltransferase SDG4 to the WOX5 promoter, thus activating WOX5 expression. Thus, analogous to its animal counterparts, SEU acts as a multi‐adaptor protein that integrates the actions of genetic and epigenetic regulators into a concerted transcriptional program to control root stem cell organizer specification. The transcriptional adaptor protein SEUSS promotes expression of the quiescent center determinant WOX5 via recruitment of the histone methyltransferase SDG4 in Arabidopsis thaliana.