REOVIRUS HEMAGGLUTININ MESSENGER-RNA CODES FOR 2 POLYPEPTIDES IN OVERLAPPING READING FRAMES
REOVIRUS HEMAGGLUTININ MESSENGER-RNA CODES FOR 2 POLYPEPTIDES IN OVERLAPPING READING FRAMES
复制标题
DOI：
10.1073/pnas.82.1.48
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
SHATKIN, AJ
中科院分区：
文献类型：
--
作者：
ERNST, H;SHATKIN, AJ
编码病毒血凝素的人呼肠孤病毒s1 mRNA还指导网织红细胞和小麦胚芽提取物中先前未识别的MW 14 kDa [千道尔顿]多肽的合成。杂交逮捕的翻译所选择的限制性片段克隆S1 DNA表明，14 kDa的多肽的合成起始于第二个八月。这是证实了由NH 2-末端序列分析。因此，14-kDa多肽的编码序列完全位于血凝素基因内，但在不同的阅读框中。虽然没有发现在病毒粒子中，14 kDa的多肽显然是在病毒感染的小鼠L细胞中形成的，通过比较细胞提取物中的[35 S]甲硫氨酸标记的多肽与相应的体外产物证明。
Human reovirus s1 mRNA, which codes for the viral hemagglutinin, also directs the synthesis of a previously unrecognized polypeptide of MW 14 kDa [kilodatons] in reticulocyte and wheat germ extracts. Hybrid-arrest of translation by selected restriction fragments of cloned S1 DNA indicated that synthesis of the 14-kDa polypeptide initiates at the 2nd AUG. This was confirmed by NH2-terminal sequence analyses. The coding sequence for the 14-kDa polypeptide thus lies entirely within the hemagglutinin gene but in a different reading frame. Although not found in virions, the 14-kDa polypeptide apparently is formed in virus-infected mouse L cells, as demonstrated by comparison of [35S]methionine-labeled polypeptides in cell extracts with the corresponding in vitro products.