Platelet aggregation in acute coronary syndromes: use of a new aggregometer with laser light scattering to assess platelet aggregability
Platelet aggregation in acute coronary syndromes: use of a new aggregometer with laser light scattering to assess platelet aggregability
复制标题
DOI：
10.1016/s0008-6363(98)00114-x
复制
发表时间：
1998-10-01
影响因子：
10.8
通讯作者：
Isshiki, T
中科院分区：
文献类型：
--
作者：
Eto, K;Takeshita, S;Isshiki, T
目的：血小板聚集与急性冠状动脉综合征的发病机制密切相关。由小于或等于100个血小板组成的小聚集体不能用采用光密度的常规聚集计定量。我们使用最近开发的基于激光散射的聚集仪，研究了急性冠状动脉综合征患者的血小板聚集性。研究方法：从39例既往未接受抗血小板或抗凝治疗的急性心肌梗死或不稳定型心绞痛患者中采集外周血样本，立即使用PA-100血小板聚集仪进行测定。14名健康志愿者的血液样本用作对照。结果：血小板聚集体的自发形成仅见于急性冠脉综合征患者。这些聚集体的尺寸较小，由小于或等于100个血小板组成（初级聚集体）。激动剂诱导的聚集由两个阶段组成。在最初的几分钟内，小聚集体的数量显著增加（初级聚集），随后是较大聚集体的增加（次级聚集）。急性冠脉综合征患者肾上腺素对原发性聚集的EC 50比对照组低近50倍（P
Objective: Platelet aggregation has been implicated in the pathogenesis of acute coronary syndromes. Small aggregates consisting of less than or equal to 100 platelets cannot be quantified with a conventional aggregometer employing optical density. Using a recently developed aggregometer based on laser light scattering, we studied platelet aggregability in patients with acute coronary syndromes. Methods: Peripheral blood samples were obtained from 39 patients with acute myocardial infarction or unstable angina who had received no prior antiplatelet or anticoagulant therapy, to be assayed immediately using a PA-100 platelet aggregometer. Blood samples from 14 healthy volunteers were used as controls. Results: Spontaneous formation of platelet aggregates was observed only in patients with acute coronary syndromes. The size of these aggregates was small, consisting of less than or equal to 100 platelets (primary aggregation). Agonist-induced aggregation consisted of two phases. In the first few minutes, the number of small aggregates increased markedly (primary aggregation), followed by an increase in larger aggregates (secondary aggregation). The EC50 of epinephrine for primary aggregation was nearly 50 times lower in acute coronary patients than in controls (P