MYB108 Acts Together with MYB24 to Regulate Jasmonate-Mediated Stamen Maturation in Arabidopsis
MYB108 Acts Together with MYB24 to Regulate Jasmonate-Mediated Stamen Maturation in Arabidopsis
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DOI：
10.1104/pp.108.132597
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发表时间：
2009-02-01
期刊：
影响因子：
7.4
通讯作者：
Browse, John
中科院分区：
文献类型：
--
作者：
Mandaokar, Ajin;Browse, John
在拟南芥（Arabidopsis thaliana）中，茉莉酸是雄蕊和花粉成熟所需的关键信号，因此也是雄性育性的关键信号。使用转录谱，我们以前已经确定了一组13个转录因子，建议参与控制茉莉酸反应在雄蕊。这一发现表明，转录级联调节许多发育和生物化学途径所需的，以确保生育能力;然而，该级联的组织目前还不清楚。在这里，我们提供了MYB 108的作用的遗传特征和映射其关系MYB 21和MYB 24，另外两个转录因子参与茉莉酸响应拟南芥雄蕊。对表达MYB 108：GUS融合蛋白的植物的转录谱分析表明，MYB 108的表达主要局限于雄蕊的孢子体组织。三个等位基因myb 108突变体表现出降低雄性育性，与延迟花药开裂，降低花粉活力，并降低相对于野生型的生殖力。这些表型都被发现在myb 108 myb 24双突变体，也有较短的雄蕊花丝加剧。在野生型和突变型花中MYB 108转录水平的测量表明，该基因的表达强烈依赖于MYB 21。总之，我们的研究结果表明，MYB 108和MYB 24具有重叠的功能，并在转录级联中作用于MYB 21的下游，介导雄蕊和花粉成熟。
In Arabidopsis (Arabidopsis thaliana), jasmonate is a key signal required for stamen and pollen maturation and thus for male fertility. Using transcriptional profiling, we have previously identified a set of 13 transcription factors that are proposed to be involved in controlling jasmonate responses in stamens. This finding suggests that a transcriptional cascade regulates the many developmental and biochemical pathways required to ensure fertility; however, the organization of this cascade is currently not understood. Here, we provide a genetic characterization of the role of MYB108 and map its relationship to MYB21 and MYB24, two other transcription factors involved in the jasmonate response in Arabidopsis stamens. Transcriptional profiling and analysis of plants expressing a MYB108:GUS fusion protein demonstrated that MYB108 expression is largely confined to sporophytic tissues of the stamen. Three allelic myb108 mutants exhibited reduced male fertility that was associated with delayed anther dehiscence, reduced pollen viability, and decreased fecundity relative to wild type. These phenotypes were all found to be exacerbated in myb108 myb24 double mutants, which also had shorter stamen filaments. Measurements of MYB108 transcript levels in wild-type and mutant flowers showed that expression of this gene is strongly dependent on MYB21. Taken together, our results indicate that MYB108 and MYB24 have overlapping functions and act downstream of MYB21 in a transcriptional cascade that mediates stamen and pollen maturation in response to jasmonate.