Treatment with camu camu (Myrciaria dubia) prevents obesity by altering the gut microbiota and increasing energy expenditure in diet-induced obese mice
Treatment with camu camu (Myrciaria dubia) prevents obesity by altering the gut microbiota and increasing energy expenditure in diet-induced obese mice
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DOI：
10.1136/gutjnl-2017-315565
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发表时间：
2019-03-01
期刊：
影响因子：
24.5
通讯作者：
Marette, Andre
中科院分区：
文献类型：
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作者：
Anhe, Fernando F.;Nachbar, Renato T.;Marette, Andre
目的水果的食用与更好的健康和更高的肠道菌群（GM）细菌多样性密切相关。卡姆果（Myrciaria dubia）是一种具有独特的植物化学特征、强抗氧化潜力和据称的抗炎潜力的亚马逊水果。设计通过使用代谢测试结合基于16 S rRNA基因的分类学分析和粪便微生物移植（FMT），我们已经评估了在高脂肪/高蔗糖（HFHS）环境下，骆驼肉粗提取物（CC）对肥胖和相关免疫代谢紊乱的作用。结果用CC处理HFHS喂养的小鼠可防止体重增加，降低脂肪积累，并减弱代谢性炎症和内毒素血症。CC处理的小鼠显示出改善的葡萄糖耐量和胰岛素敏感性，并且还完全防止肝脂肪变性。这些作用与CC处理小鼠棕色脂肪组织（BAT）中能量消耗增加和解偶联蛋白1 mRNA表达上调有关，这与膜胆汁酸（BA）受体TGR 5的mRNA表达密切相关。此外，CC处理的小鼠显示出血浆BA池大小和组成的改变以及GM的剧烈变化（例如，嗜粘蛋白阿克曼氏菌的大量繁殖和乳酸杆菌的大量减少）。无菌（GF）重建的小鼠与GM的CC治疗的小鼠体重减轻，并表现出更高的能量消耗比GF-小鼠殖民地的FM HFHS controls.Conclusion我们的研究结果表明，CC防止内脏和肝脏脂肪沉积通过BAT激活和增加能量消耗，一种机制，是依赖于GM和连接到BA池的大小和组成的重大变化。
Objective T he consumption of fruits is strongly associated with better health and higher bacterial diversity in the gut microbiota (GM). Camu camu (Myrciaria dubia) is an Amazonian fruit with a unique phytochemical profile, strong antioxidant potential and purported anti-inflammatory potential.Design By using metabolic tests coupled with 16S rRNA gene-based taxonomic profiling and faecal microbial transplantation (FMT), we have assessed the effect of a crude extract of camu camu (CC) on obesity and associated immunometabolic disorders in high fat/ high sucrose (HFHS)-fed mice.Results T reatment of HFHS-fed mice with CC prevented weight gain, lowered fat accumulation and blunted metabolic inflammation and endotoxaemia. CC-treated mice displayed improved glucose tolerance and insulin sensitivity and were also fully protected against hepatic steatosis. These effects were linked to increased energy expenditure and upregulation of uncoupling protein 1 mRNA expression in the brown adipose tissue (BAT) of CC-treated mice, which strongly correlated with the mRNA expression of the membrane bile acid (BA) receptor TGR5. Moreover, CC-treated mice showed altered plasma BA pool size and composition and drastic changes in the GM (eg, bloom of Akkermansia muciniphila and a strong reduction of Lactobacillus). Germ-free (GF) mice reconstituted with the GM of CCtreated mice gained less weight and displayed higher energy expenditure than GF-mice colonised with the FM of HFHS controls.Conclusion Our results show that CC prevents visceral and liver fat deposition through BAT activation and increased energy expenditure, a mechanism that is dependent on the GM and linked to major changes in the BA pool size and composition.