Effects of caffeic acid on behavioral parameters and on the activity of acetylcholinesterase in different tissues from adult rats
Effects of caffeic acid on behavioral parameters and on the activity of acetylcholinesterase in different tissues from adult rats
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DOI：
10.1016/j.pbb.2012.09.006
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发表时间：
2012-12-01
影响因子：
3.6
通讯作者：
Chitolina Schetinger, Maria Rosa
中科院分区：
文献类型：
--
作者：
Anwar, Javed;Spanevello, Roselia Maria;Chitolina Schetinger, Maria Rosa
乙酰胆碱酯酶（AChE）广泛分布于神经和非神经组织中，对机体的生理活动起着重要的调节作用。咖啡酸是一种酚类化合物，具有抗炎和神经保护作用。本研究的目的是调查在体外和体内是否咖啡酸改变乙酰胆碱酯酶活性和行为参数的大鼠。在体外研究中，使用浓度为0、0.1、0.5、1.0、1.5和2 mM的咖啡酸。对于体内研究，评价了五组：组I（对照）;组II（芥花油）;组III（10 mg/kg咖啡酸）;组IV（50 mg/kg咖啡酸）和组V（100 mg/kg咖啡酸）。将咖啡酸稀释在芥花油中并施用30天。在体外实验中，不同浓度的咖啡酸可增加大脑皮层、小脑、下丘脑、全血和淋巴细胞中AChE的活性。在肌肉中，该化合物在0.5、1.0、1.5的浓度下引起AChE活性的抑制。和2 mM时，与对照相比（P
Acetylcholinesterase (AChE) is distributed throughout the body in both neuronal and non-neuronal tissues and plays an important role in the regulation of physiological events. Caffeic acid is a phenolic compound that has anti-inflammatory and neuroprotective properties. The aim of this study was to investigate in vitro and in vivo whether caffeic acid alters the AChE activity and behavioral parameters in rats. In the in vitro study, the concentrations of 0, 0.1, 0.5, 1.0, 1.5, and 2 mM of caffeic acid were used. For the in vivo study, five groups were evaluated: group I (control); group II (canola oil), group III (10 mg/kg of caffeic acid); group IV (50 mg/kg of caffeic acid) and group V (100 mg/kg of caffeic acid). Caffeic acid was diluted in canola oil and administered for 30 days. In vitro, the caffeic acid increased the AChE activity in the cerebral cortex, cerebellum, hypothalamus, whole blood, and lymphocytes at different concentrations. In muscle, this compound caused an inhibition in the AChE activity at concentrations of 0.5, 1.0, 1.5. and 2 mM when compared to the control (P