Purification and partial sequence analysis of human T-cell growth factor.
Purification and partial sequence analysis of human T-cell growth factor.
复制标题
人 T 细胞生长因子的纯化和部分序列分析。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
V. Chowdhry
中科院分区：
文献类型：
--
作者：
R. Robb;R. Kutny;V. Chowdhry
A murine monoclonal antibody directed against human T-cell growth factor (TCGF) from the JURKAT cell line was used for affinity column purification of the factor. Bound TCGF was eluted nearly quantitatively at low pH, and the recovered factor appeared homogeneous by two-dimensional gel electrophoresis. The molecule is markedly hydrophobic, with a high content of leucine. A single NH2-terminal sequence of 36 residues was obtained by automated Edman degradation, further supporting the homogeneity of the material. Thus, significant quantities of purified TCGF have been prepared in a single step, making possible detailed analysis of its molecular structure and biological role.
