JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time
JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time
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DOI：
10.1111/tpj.13623
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发表时间：
2017-09-01
期刊：
影响因子：
7.2
通讯作者：
Raina, Ramesh
中科院分区：
文献类型：
--
作者：
Dutta, Aditya;Choudhary, Pratibha;Raina, Ramesh
已知组蛋白甲基化动态调节多种发育和生理过程。组蛋白甲基标记由甲基转移酶写入并由去甲基酶清除，并导致染色质结构的修饰以抑制或激活转录。然而，很少有人知道组蛋白甲基化如何调节植物的防御机制和开花时间。在这里，我们报告的JmjC结构域含有蛋白27（JMJ 27），拟南芥JHDM 2（JmjC结构域含有组蛋白去甲基化酶2）家族蛋白，调节防御病原体和开花时间的特性。JMJ 27是一种核蛋白，含有锌指基序和催化JmjC结构域，具有保守的Fe（II）和α-酮戊二酸结合位点，并在体外和体内均显示H3 K9 me 1/2脱甲基酶活性。JMJ 27是响应于毒性假单胞菌病原体而诱导的，并且是针对这些病原体的抗性所需的。JMJ 27是WRKY 25（防御阻遏物）的负调节剂和几种病程相关（PR）蛋白的正调节剂。此外，JMJ 27功能的丧失导致早期开花。JMJ 27负调节主要开花调节因子CONSTANS（CO），正调节FLOWERY LOCUS C（FLC）。综上所述，我们的研究结果表明，JMJ 27功能作为组蛋白去甲基化酶调节生理（防御）和发育（开花时间）过程中拟南芥。
Histone methylation is known to dynamically regulate diverse developmental and physiological processes. Histone methyl marks are written by methyltransferases and erased by demethylases, and result in modification of chromatin structure to repress or activate transcription. However, little is known about how histone methylation may regulate defense mechanisms and flowering time in plants. Here we report characterization of JmjC DOMAIN-CONTAINING PROTEIN 27 (JMJ27), an Arabidopsis JHDM2 (JmjC domain-containing histone demethylase 2) family protein, which modulates defense against pathogens and flowering time. JMJ27 is a nuclear protein containing a zinc-finger motif and a catalytic JmjC domain with conserved Fe(II) and alpha-ketoglutarate binding sites, and displays H3K9me1/2 demethylase activity both in vitro and in vivo. JMJ27 is induced in response to virulent Pseudomonas syringae pathogens and is required for resistance against these pathogens. JMJ27 is a negative modulator of WRKY25 (a repressor of defense) and a positive modulator of several pathogenesis-related (PR) proteins. Additionally, loss of JMJ27 function leads to early flowering. JMJ27 negatively modulates the major flowering regulator CONSTANS (CO) and positively modulates FLOWERING LOCUS C (FLC). Taken together, our results indicate that JMJ27 functions as a histone demethylase to modulate both physiological (defense) and developmental (flowering time) processes in Arabidopsis.