ENTERIC VIRUSES AND DIARRHEA IN HIV-INFECTED PATIENTS
ENTERIC VIRUSES AND DIARRHEA IN HIV-INFECTED PATIENTS
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DOI：
10.1056/nejm199307013290103
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发表时间：
1993-07-01
影响因子：
158.5
通讯作者：
BRYAN, RT
中科院分区：
文献类型：
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作者：
GROHMANN, GS;GLASS, RI;BRYAN, RT
背景。腹泻经常发生在获得性免疫缺陷综合征患者中，但其原因往往尚不清楚。我们使用了一组诊断方法来确定哪些病毒是人类免疫缺陷病毒（HIV）感染者腹泻的病因。粪便和血清标本来自亚特兰大一项纵向研究的hiv感染患者。采用电子显微镜、聚丙烯酰胺凝胶电泳和酶免疫分析法对腹泻患者和对照组非腹泻患者的粪便标本进行轮状病毒、肠腺病毒、杯状病毒、小核糖核酸病毒和星状病毒的筛选。检测配对血清样本对诺瓦克病毒和小核糖核酸病毒的抗体反应。109例腹泻患者的粪便标本中有35%检测到病毒，而113例非腹泻患者的粪便标本中只有12%检测到病毒
Background. Diarrhea occurs frequently among persons with the acquired immunodeficiency syndrome, but the cause often remains unknown. We used a group of diagnostic assays to determine which viruses were etiologic agents of diarrhea in a group of persons infected with the human immunodeficiency virus (HIV).Methods. Stool and serum specimens were obtained from HIV-infected patients enrolled in a longitudinal study in Atlanta. Fecal specimens from patients with diarrhea and from control patients without diarrhea were screened by electron microscopy, polyacrylamide-gel electrophoresis, and enzyme immunoassays for rotaviruses, enteric adenoviruses, caliciviruses, picobirnaviruses, and astroviruses. Paired serum samples were tested for antibody responses to Norwalk virus and picobirnavirus.Results. Viruses were detected in 35 percent of 109 fecal specimens from patients with diarrhea but in only 12 percent of 113 specimens from those without diarrhea (P