Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells.
Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells.
复制标题
PAX3和PAX7在成年肌肉祖细胞中具有明显的重叠功能。
DOI：
10.1083/jcb.200508044
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发表时间：
2006-01-02
影响因子：
7.8
通讯作者：
Buckingham, Margaret
中科院分区：
文献类型：
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作者：
Relaix, Frederic;Montarras, Didier;Zaffran, Stephane;Gayraud-Morel, Barbara;Rocancourt, Didier;Tajbakhsh, Shahragim;Mansouri, Ahmed;Cumano, Ana;Buckingham, Margaret
出生后骨骼肌的生长和修复依赖于以Pax7表达为特征的卫星细胞。我们发现Pax3， Pax7的旁系蛋白，也存在于许多骨骼肌的静止和激活的卫星细胞中。Pax3和-7的显性阴性形式均抑制MyoD，但不干扰另一种成肌决定因子Myf5的表达，而Myf5与Pax3/7一起调节这些细胞的成肌分化。在Pax7突变体中，在表达Pax7和不表达Pax7的肌肉中，卫星细胞逐渐丢失。我们表明，这是由卫星细胞死亡引起的，对细胞周期有影响。在卫星细胞培养中操纵这些因子的显性阴性形式表明，Pax3不能取代Pax7的抗凋亡功能。这些发现强调了细胞存活在控制成体组织干细胞群中的重要性，并证明了上游因素在此背景下的作用。
The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context.