Preparation of W/O/W microcapsule containing enzyme without alcohol
Preparation of W/O/W microcapsule containing enzyme without alcohol
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DOI：
10.1016/j.colsurfa.2009.03.057
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发表时间：
2009-09
期刊：
影响因子：
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通讯作者：
Takayuki Narita;T. Kishigawa;Yasunobu Tagami;Y. Oishi
中科院分区：
文献类型：
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作者：
Takayuki Narita;T. Kishigawa;Yasunobu Tagami;Y. Oishi
提出了一种制备W/O/W型酶微胶囊的新方法。在该方法中，使用气相代替在用于制备W/O/W型微胶囊的溶剂改变方法中常规使用的醇。作为本研究的模型系统，我们制备了葡萄糖氧化酶封装的聚（L-赖氨酸-盐-对苯二甲酸）微胶囊，其体积可以在葡萄糖的存在下减少，并评估葡萄糖对收缩过程的影响。为了估计微胶囊中酶的相对浓度，提出了一个简单的模型，假设微胶囊膜的尺寸和电荷密度的变化率的线性依赖性。观察到的收缩微胶囊的直径与理论值一致。通过拟合所观察到的微胶囊大小的时间过程的理论方程，得到两个时间常数，都与微胶囊中的相对酶浓度成反比。从通过新方法和常规方法制备的微胶囊的它们的常数的比较中，显而易见的是，通过新方法制备的含有葡萄糖氧化酶的微胶囊表现出比在溶剂改变过程中使用乙醇的常规方法的情况高约1.5倍的酶活性。
We proposed a novel method for the preparation of W/O/W type microcapsules containing enzymes. In this method, a gas phase was used in place of alcohol that has been conventionally used in solvent change processes for the preparation of W/O/W type microcapsules. As a model system for this study, we prepared glucose-oxidase encapsulated poly(l-lysine-alt-terephthalic acid) microcapsules whose volume can be decreased in the presence of glucose and evaluate the effect of glucose on the shrinking process. To estimate the relative concentration of enzyme in microcapsule, a simple model was proposed on assumption of linear dependence of the rate of change in the size and in the charge density of the microcapsule membrane. The diameters of the shrunk microcapsule observed were consistent with the theoretical value. By a fitting of the observed time course of the microcapsule size to the theoretical equations, two time constants were obtained, both related inversely to the relative enzyme concentration in the microcapsules. From the comparison of their constants for microcapsules prepared by the novel and conventional method, it became apparent that the glucose-oxidase containing microcapsule prepared by the novel method exhibited about 1.5 times higher enzyme activity of the case for the conventional method using ethanol in the solvent change processes.