Effect of exposure to hyperbaric oxygen on diabetes-induced cataracts in mice
Effect of exposure to hyperbaric oxygen on diabetes-induced cataracts in mice
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DOI：
10.1111/j.1753-0407.2011.00150.x
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发表时间：
2011-12-01
影响因子：
4.5
通讯作者：
Ishihara, Akihiko
中科院分区：
文献类型：
--
作者：
Nagatomo, Fumiko;Roy, Roland R.;Ishihara, Akihiko
背景：患有 2 型糖尿病的动物生长相关的血糖水平升高受到 1.25 个绝对大气压 (ata)、36% 氧气的中等高压暴露的抑制，可能是由于氧化代谢的增加。然而，目前还没有关于中度高压氧（HBO）对糖尿病引起的白内障的影响的数据。方法：患有2型糖尿病和白内障的4周龄小鼠暴露于1.25 ata和36%氧气中，每天6小时，持续12周，然后在正常条件下暴露于1 ata和21%氧气中，持续16周（白内障+高压组）。将这些小鼠在 4、8、12、16 和 32 周龄时的血糖和活性氧代谢物衍生物 (dROM) 的水平（用作氧化应激的指标）以及晶状体的混浊度与对照组和未暴露于 HBO 的糖尿病（白内障组）小鼠进行比较。 结果：白内障 + 高压氧组在 12、16 和 16 岁时的非空腹和空腹血糖水平较低。与年龄匹配的白内障组相比，年龄为 32 周。 16周龄和32周龄时，白内障+高压组的dROMs水平低于年龄匹配的白内障组。 12、16和32周龄时，白内障+高压组的晶状体周边和中心区域的浑浊度低于同龄白内障组。结论：1.25 ata和36%氧气的高压暴露可延缓2型糖尿病小鼠白内障的发生和进展。
Background: The growth-associated increase in the blood glucose level of animals with Type 2 diabetes is inhibited by moderate hyperbaric exposure at 1.25 atmospheres absolute (ata) with 36% oxygen, presumably due to an increase in oxidative metabolism. However, there are no data available regarding the effect of moderate hyperbaric oxygen (HBO) on diabetes-induced cataracts.Methods: Four-week-old mice with Type 2 diabetes and cataracts were exposed to 1.25 ata with 36% oxygen, 6 h daily, for 12 weeks, followed by normal conditions at 1 ata with 21% oxygen for 16 weeks (cataract + hyperbaric group). Levels of blood glucose and derivatives of reactive oxygen metabolites (dROMs), used as an index of oxidative stress, and the turbidities of the lenses from these mice at 4, 8, 12, 16, and 32 weeks of age were compared with those of control and diabetic (cataract group) mice not exposed to HBO.Results: Non-fasting and fasting blood glucose levels were lower in the cataract + hyperbaric group at 12, 16, and 32 weeks of age than in the age-matched cataract group. The levels of dROMs were lower in the cataract + hyperbaric group at 16 and 32 weeks of age than in the age-matched cataract group. The turbidities of the peripheral and central regions of the lenses were lower in the cataract + hyperbaric group at 12, 16, and 32 weeks of age than in the age-matched cataract group.Conclusions: Hyperbaric exposure at 1.25 ata with 36% oxygen delays cataract development and progression in mice with Type 2 diabetes.