Amlodipine Improves Spinal Cord Injury Repair by Inhibiting Motoneuronal Apoptosis Through Autophagy Upregulation.
Amlodipine Improves Spinal Cord Injury Repair by Inhibiting Motoneuronal Apoptosis Through Autophagy Upregulation.
复制标题
氨氯地平通过上调自噬抑制运动神经元凋亡来改善脊髓损伤修复
作者：
研究设计.通过建立脊髓腹侧细胞（VSC4.1）缺氧缺糖模型和脊髓损伤（SCI）小鼠模型，研究黄芪（AM）对脊髓损伤（SCI）和自噬的影响。Objective.确定AM在SCI修复过程中通过上调自噬的神经保护作用。背景数据总结。AM是一种抗高血压药物，在一些研究中显示其抑制神经元凋亡并在多种中枢神经系统疾病中发挥神经保护作用。然而，其对SCI的影响尚未研究。自噬可以抑制细胞凋亡，促进脊髓损伤的修复。然而，AM在自噬中的作用仍不清楚。方法.本研究通过组织学、行为学、显微镜、免疫荧光和Western印迹分析，分别研究了C57 BL/6雌性小鼠腹侧脊髓细胞和损伤脊髓中AM、凋亡和自噬之间的关系。结果我们发现AM能抑制体外培养的运动神经元凋亡。此外，AM通过上调自噬和减轻细胞凋亡，神经元丢失和脊髓损伤后促进运动恢复。结论AM通过上调自噬抑制脊髓运动神经元凋亡，促进脊髓损伤的恢复。
Study Design. The effect of amlodipine (AM) on spinal cord injury (SCI) and autophagy was researched by establishing ventral spinal cord cells (VSC4.1) oxygen and glucose deprivation model and SCI mice model. Objective. To determine the neuroprotective effects of AM by upregulating autophagy during SCI repair. Summary of Background Data. AM, an antihypertensive medication, has been shown in several studies to inhibit neuronal apoptosis and exert neuroprotective effects in various central nervous system diseases. However, its effects on SCI are unexplored. Autophagy could inhibit cell apoptosis, which has been shown to promote SCI repair. However, the role of AM in autophagy remains unclear. Methods. We examined the relationship between AM, apoptosis, and autophagy in ventral spinal cord cells and the injured spinal cords of C57BL/6 female mice respectively, following histological, behavioral, microscopic, immunofluorescence, and western blotting analyses. Results. We found that AM could inhibit motor neuronal apoptosis in vitro. Furthermore, AM promoted locomotor recovery by upregulating autophagy and alleviating apoptosis, neuronal loss, and spinal cord damage after SCI. Conclusion. AM inhibited motoneuronal apoptosis by upregulating autophagy to improve SCI recovery.