A MYCN-amplified cell line derived from a long-term event-free survivor among our sixteen established neuroblastoma cell lines.
A MYCN-amplified cell line derived from a long-term event-free survivor among our sixteen established neuroblastoma cell lines.
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MYCN 扩增细胞系源自我们已建立的 16 种神经母细胞瘤细胞系中的长期无事件幸存者。
DOI：
10.1016/j.canlet.2012.12.011
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发表时间：
2013
期刊：
影响因子：
9.7
通讯作者：
Nakagawara A.
中科院分区：
文献类型：
--
作者：
Sugimoto T;Gotoh T;Yagyu S;Kuroda H;Iehara T;Hosoi H;Ohta S;Ohira M;Nakagawara A.
尽管已经建立了超过110种神经母细胞瘤（NB）细胞系，但既没有关于建立NB细胞系成功率的报道，也没有来自长期存活者的NB细胞系的详细记录。我们试图从 114 名患者身上建立 NB 细胞系。从 12 名患者中建立了 16 种 NB 细胞系。早期患者细胞系建立成功率为1.4%（1/70），晚期患者为25.0%（11/44），各期患者细胞系建立成功率为10.5%（12/114）。这12名患者中有11人最终死亡。幸存的患者处于 MYCN 扩增的第 4 期，在完成治疗后 19 年没有发生任何事件。 TK患者的血清MYCN DNA水平在治疗前很高，化疗后下降，至今一直维持在正常水平。使用NB特异性cDNA微阵列对原发肿瘤和K-N-TK细胞系的基因表达谱进行分析，结果表明5年生存的可能性极低。基于微阵列的比较基因组杂交 (CGH) 分析表明，该细胞系的基因组畸变谱并不常见，存在 MYCN 扩增、17q 增益和 11q 缺失。由无事件幸存者建立的独特 KP-N-TK 细胞系将成为研究患者如何在预后极差的肿瘤中生存的有用工具。
Although more than 110 neuroblastoma (NB) cell lines have been established, there have been neither reports on the rate of success to establish NB cell lines, nor well-documented NB cell lines from long-term-survivors. We attempted to establish NB cell lines from 114 patients. Sixteen NB cell lines were established from 12 patients. The success rates to establish cell lines were 1.4% (1/70) from patients in early stages, 25.0% (11/44) from those in advanced stages, and 10.5% (12/114) from those in all stages respectively. Eleven of these 12 patients eventually died. The surviving patient, who was in stage 4 with MYCN-amplification, has been event-free for 19years after completing therapy. The serum MYCN DNA level in patient TK was very high before therapy, decreased after chemotherapy, and has remained at the normal levels until now. The gene expression profiling of the primary tumor and the K-N-TK cell line was analyzed with an NB-specific cDNA microarray, and indicated that the probability of 5-year survival was extremely low. Microarray-based comparative genomic hybridization (CGH) analysis indicated that genomic aberration profiles of the cell line were uncommon, with MYCN amplification, 17q gain and 11q loss. A unique KP-N-TK cell line, established from an event-free survivor, will be a useful tool for investigating how a patient can survive a tumor with an extremely poor prognosis.