Biodegradation of isoproturon by Pseudoxanthomonas sp isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar
Biodegradation of isoproturon by Pseudoxanthomonas sp isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar
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DOI：
10.1007/s13205-016-0505-8
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发表时间：
2016-09-02
期刊：
影响因子：
2.8
通讯作者：
Rai, J. P. N.
中科院分区：
文献类型：
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作者：
Giri, Krishna;Pandey, Shailseh;Rai, J. P. N.
从潘特纳加尔Tarai农业生态系统施用除草剂的麦田中分离到一种革兰氏阴性、棒状、利用异丙隆（伊普）的细菌。基于16 S rRNA序列的系统发育分析表明，该菌株可能是假单胞菌属中的一个独立种。该分离物与日本假黄单胞菌（Pseudoxanthomonasjaponensis）有近缘种（95%相似性），命名为K2.用KB 009 Part A和B HiCarbohydrate（TM）试剂盒对细菌分离物的氧化酶、过氧化氢酶和20种碳水化合物显示阳性反应。降解实验进行了使用200毫克升（-1）初始伊普作为碳源，在不同的pH值和温度。K2在pH 7.0和30 ℃下观察到最大伊普降解，而在pH 6.5和25 ℃下观察到最小降解。在最佳条件下，添加葡萄糖沿着伊普作为辅助碳源，与不添加葡萄糖的伊普降解相比，IPU降解提高了4.72%。4-在培养基中检测到异丙基苯胺作为降解副产物。本研究证明了一种新的细菌分离物K2的伊普代谢能力，可以是一个更好的选择，IPU污染的网站修复。
A gram-negative, rod-shaped, isoproturon (IPU) utilizing bacterium was isolated from herbicide-applied wheat fields of Tarai agro-ecosystem, Pantnagar. The phylogenetic sequence analysis based on 16S rRNA sequence revealed that the isolate could be a distinct species within the genus Pseudomonas. The isolate was a close relative of Pseudoxanthomonas japonensis (95 % similarity) and designated as K2. The bacterial isolate showed positive reaction for oxidase, catalase, and 20 carbohydrates using KB009 Part A and B HiCarbohydrate (TM) Kit. Degradation experiments were conducted using 200 mg l(-1) initial IPU as a source of carbon at different pH and temperatures. Maximum IPU degradation by K2 was observed at pH 7.0 and 30 degrees C, while least degradation at 6.5 pH and 25 degrees C. Addition of dextrose along with IPU as an auxiliary carbon source increased IPU degradation by 4.72 %, as compared to the IPU degradation without dextrose under optimum conditions. 4-isopropylaniline was detected as a degradation by-product in the medium. The present study demonstrated the IPU metabolizing capacity of a novel bacterial isolate K2 that can be a better choice for the remediation of IPU-contaminated sites.