Mechanisms of neurodegeneration after severe hypoxic-ischemic injury in the neonatal rat brain
Mechanisms of neurodegeneration after severe hypoxic-ischemic injury in the neonatal rat brain
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DOI：
10.1016/j.brainres.2015.10.020
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发表时间：
2015-12-10
期刊：
影响因子：
2.9
通讯作者：
Yager, Jerome Y.
中科院分区：
文献类型：
--
作者：
Askalan, Rand;Gabarin, Nadia;Yager, Jerome Y.
目的：细胞凋亡与轻度 - 中度缺血性损伤有关。严重缺血性脑的细胞死亡途径尚不明确。我们试图确定细胞凋亡在严重缺血的未成熟脑中的作用。
方法：7日龄大鼠被随机分为轻度 - 中度或重度脑缺氧缺血（HI）组。结扎右侧颈总动脉后，轻度 - 中度HI组动物缺氧90分钟，重度HI组动物缺氧180分钟。使用NIS Elements软件在H&E染色的脑切片中测量核心区和半暗带梗死区。对脑切片进行半胱天冬酶 - 3（caspase - 3）、凋亡诱导因子（AIF）和受体相互作用蛋白激酶3（RIP3）免疫染色。对两组的阳性细胞数量进行计数并比较。
结果：与中度损伤的脑相比，重度损伤脑中核心区在缺血性病变中所占比例显著更高（P
Purpose: Apoptosis is implicated in mild-moderate ischemic injury. Cell death pathways in the severely ischemic brain are not characterized. We sought to determine the role of apoptosis in the severely ischemic immature brain.Methods: Seven-day old rats were randomly assigned to mild-moderate or severe cerebral hypoxia-ischemia (HI) group. After ligating the right common carotid artery, animals were subjected to hypoxia for 90 min in the mild-moderate HI or 180 min in the severe HI. The core and pen-infarct area were measured in H&E stained brain sections using NIS Elements software. Brain sections were processed for caspase-3, AIF and RIP3 immuno-staining. Number of positive cells were counted and compared between the two groups.Results: The core constituted a significantly higher proportion of the ischemic lesion in the severely compared to the moderately injured brain (P