MYB transcription factors orchestrating the developmental program of xylem vessels in Arabidopsis roots
MYB transcription factors orchestrating the developmental program of xylem vessels in Arabidopsis roots
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DOI：
10.5511/plantbiotechnology.27.267
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发表时间：
2010-06
影响因子：
1.6
通讯作者：
Yoshimi Nakano;N. Nishikubo;Nadia Goué;M. Ohtani;M. Yamaguchi;Y. Katayama;T. Demura
中科院分区：
文献类型：
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作者：
Yoshimi Nakano;N. Nishikubo;Nadia Goué;M. Ohtani;M. Yamaguchi;Y. Katayama;T. Demura
木质部导管分子在陆地植物的水分和养分输送中起着重要作用。在体外培养系统中，继代培养的拟南芥细胞被诱导分化为木质部导管分子，通过基因芯片分析揭示了全面的基因表达谱，从而鉴定了许多编码转录因子的基因，包括两个转录关键调控因子，VASCUAR相关的NAC DOMAIN6(VND6)和VND7。在此，我们分析了编码MYB转录因子MYB20、43、46、52、63、83、85、99、103和118的10个基因的详细表达模式和功能，这些基因在体外导管分子分化过程中表达显著上调。其中有6个在拟南芥根木质部导管分化过程中表现出特异性表达。此外，MYB46和MYB83被证明上调了这些MYB基因中的5个，MYB43、52、63、85和103。我们的结果表明，在根中木质部导管分子分化过程中，几个MYB基因组成了一个转录网络。
Xylem vessel elements play an important role in conducting water and nutrient in land plants. The in vitro culture system, in which subcultured Arabidopsis cells are induced to transdifferentiate into xylem vessel elements, was used to uncover the comprehensive gene expression profile by microarray analysis, which resulted in the identification of many genes encoding transcription factors including two transcriptional key regulators, VASCUAR-RELATED NAC DOMAIN6 (VND6) and VND7. Here we analyze the detailed expression pattern and function of ten genes encoding MYB transcription factors, MYB20, 43, 46, 52, 63, 83, 85, 99, 103, and 118, with the significantly up-regulated expression during the in vitro vessel element differentiation. Of these, six exhibited the specific expression in differentiating xylem vessels in Arabidopsis roots. In addition, MYB46 and MYB83 were shown to upregulate five of these MYB genes, MYB43, 52, 63, 85, and 103. Our results suggest that several MYB genes comprise a transcriptional network during xylem vessel element differentiation in roots.