Encapsulation of Albumin in Organic Nanotube Channel: Structural Investigation by Small-Angle X-ray Scattering
Encapsulation of Albumin in Organic Nanotube Channel: Structural Investigation by Small-Angle X-ray Scattering
复制标题
有机纳米管通道中白蛋白的封装：小角 X 射线散射的结构研究
DOI：
10.1021/acsabm.9b00047
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发表时间：
2019
影响因子：
4.7
通讯作者：
Sakurai Kazuo
中科院分区：
文献类型：
--
作者：
Kawauchi Hiroki;Takahashi Rintaro;Kameta Naohiro;Fujii Shota;Lee Ji Ha;Shimizu Toshimi;Sakurai Kazuo
已知合理设计的bola两亲物自组装成纳米管结构。在这里，我们报告的纳米管和牛血清白蛋白（BSA）之间的包合物的形成。该复合物的形成通过紫外（UV）吸收和电泳光散射（ELS）证实。通过透射电子显微镜（TEM）和小角X射线散射（SAXS）研究了不同BSA与纳米管混合比例的结构。用我们提出的模型进行的结构分析表明，BSA分子被包含在纳米管的内部空间中，并保持其管状结构。
Rationally designed bolaamphiphiles are known to self-assemble into nanotube structures. Herein, we report the formation of an inclusion complex between a nanotube and bovine serum albumin (BSA). This complex formation was confirmed by ultraviolet (UV) absorbance and electrophoretic light scattering (ELS). The structure for different mixing ratios of BSA to the nanotube was also investigated by transmission electron microscopy (TEM) and small-angle X-ray scattering (SAXS). The structural analysis with our proposed model has revealed that the BSA molecules were contained within the internal space of the nanotube with maintenance of its tubular structure.