Measurements of Cardiac Ion Channel Subunits in the Chronic Atrioventricular Block Dog
Measurements of Cardiac Ion Channel Subunits in the Chronic Atrioventricular Block Dog
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DOI：
10.1254/jphs.11019sc
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发表时间：
2011-05-01
影响因子：
3.5
通讯作者：
Sugiyama, Atsushi
中科院分区：
文献类型：
--
作者：
Takahara, Akira;Wagatsuma, Hirotaka;Sugiyama, Atsushi
慢性房室传导阻滞（CAVB）犬已被广泛用作致心律失常动物模型。采用实时荧光定量PCR技术检测正常和CAVB犬心室肌组织中K（+）和Ca（2+）通道的mRNA水平。与完整心脏相比，CAVB心脏中KvLQT 1和MiRP 1的mRNA水平显著降低，而其他K（+）和Ca（2+）通道亚基的mRNA水平无显著差异。对慢性心动过缓相关病理生理学的适应可能降低了心脏K（+）通道的mRNA水平，这可能部分解释了该模型的致心律失常特性。
The chronic atrioventricular block (CAVB) dog has been widely used as an in vivo proarrhythmia model. mRNA levels of K(+) and Ca(2+) channels in the isolated ventricular tissues from normal and CAVB dogs were assayed using a real-time PCR. The mRNA levels of KvLQT1 and MiRP1 were significantly less in the CAVB heart compared with those in the intact heart, whereas no significant difference was detected in the mRNA levels of other K(+)- or Ca(2+)-channel subunits. Adaptation against chronic bradycardia-related pathophysiology may have decreased the mRNA levels of cardiac K(+) channels, which may partly explain the arrhythmogenic property of this model.