GLYCEROLIPID BIOSYNTHESIS IN RAT ADIPOSE-TISSUE .4. INFLUENCE OF ADIPOSE-CELL SIZE AND SITE OF ADIPOSE-TISSUE ON TRIACYLGLYCEROL FORMATION IN LEAN AND OBESE RATS
GLYCEROLIPID BIOSYNTHESIS IN RAT ADIPOSE-TISSUE .4. INFLUENCE OF ADIPOSE-CELL SIZE AND SITE OF ADIPOSE-TISSUE ON TRIACYLGLYCEROL FORMATION IN LEAN AND OBESE RATS
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DOI：
10.1042/bj1700153
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发表时间：
1978-01-01
影响因子：
4.1
通讯作者：
JAMDAR, SC
中科院分区：
文献类型：
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作者：
JAMDAR, SC
以[14 C]甘油3-磷酸、[14 C]葡萄糖或[14 C]乙酸酯为底物，比较了瘦大鼠和肥胖大鼠不同脂肪库的脂质形成率。在瘦的动物中，皮下脂肪组织的脂质合成率显着低于肾周和性腺脂肪组织。在肥胖动物中，脂质合成的速率显著更高，并且从一个脂肪库到另一个没有变化。在肥胖动物的饮食限制期间，瘦大鼠和肥胖大鼠之间脂质形成速率的差异消失。分离的脂肪细胞制备物未反映脂肪器官的真实代谢活性，因为该制备物主要来源于代谢活性低于较大脂肪细胞的较小脂肪细胞。最好使用整个组织制备物来测量脂肪生成和酯化反应，因为这些测量代表了较大和较小脂肪细胞对脂质形成的贡献。
The rates of lipid formation were compared in different fat-depots from lean and obese rats by using [14C]glycerol 3-phosphate, [14C]glucose or [14C]acetate as substrates. In lean animals, subcutaneous adipose tissue showed significantly lower rates of lipid synthesis than did perirenal and gonadal fat-tissue. In obese animals, the rates of lipid synthesis were significantly higher and did not vary from one fat-depot to another. Differences in the rates of lipid formation between lean and obese rats disappeared during dietary restriction of obese animals. The isolated adipocyte preparation did not reflect the true metabolic activity of the adipose organ, since this preparation was mainly derived from smaller adipocytes that were metabolically less active than larger adipocytes. It is better to use whole tissue preparations to measure lipogenesis and esterification reactions, because these measurements represent the contribution of both larger and smaller adipocytes towards lipid formation.